当前位置: 首页 > 文章 > 小鼠肝炎病毒以不同途径感染4个品系小鼠的比较 上海交通大学学报(农业科学版) 2005,23 (2) 125-128+133
Position: Home > Articles > Comparison for Infection of Mouse Hepatitis Virus via Various Routes Journal of Shanghai Jiaotong University(Agricultural Science) 2005,23 (2) 125-128+133

小鼠肝炎病毒以不同途径感染4个品系小鼠的比较

作  者:
王英;邵伟娟;高俊;谢建云;孙凤萍;蒋凤英;李春华;胡建华;高诚
单  位:
上海市农业科学院畜牧兽医研究所;上海市实验动物研究中心
关键词:
小鼠肝炎病毒;近交系小鼠;免疫缺陷小鼠;RT-PCR
摘  要:
用小鼠肝炎病毒A59株以滴鼻、灌胃、腹腔注射3种途径人工感染近交系小鼠(BALB/c小鼠、C57BL小鼠)、免疫缺陷小鼠(NIH-III小鼠、BALB/c-nu/nu小鼠),接种病毒后3、5、7、14、21、28d分别取各鼠的肝脏,提取肝脏总RNA,用RT-PCR方法检测小鼠肝炎病毒的感染情况。结果显示:腹腔注射病毒的小鼠在肝脏中能最早检测出病毒,其次是灌胃,滴鼻则最晚;另外,近交系小鼠及免疫缺陷小鼠感染后3d在肝脏中检测出病毒,而转基因小鼠则在感染后7d在肝脏中检测出病毒。
译  名:
Comparison for Infection of Mouse Hepatitis Virus via Various Routes
作  者:
WANG Ying1, SHAO Wei- juan2, GAO Jun1, XIE Jian- yun2, SUN Feng- ping1, JIANG Feng- ying1, LI Chun- hua1, HU Jian- hua2, GAO Cheng2 ( 1.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106; 2.Shanghai Laboratory Animals Research Centre, Shanghai 200032,China)
关键词:
mouse hepatitis virus; inbred strain mice; immunodificient mice; R T- PCR
摘  要:
Inbred strain mice (BALB/c,C57BL) and immunodificient mice (NIH- I II, BALB/c- nu/nu) were inoculated with MHV- A59 via three different routes (n asal, oral, belly). Total RNA were extracted from the liver of mice inoculated o n the 3rd, 5th, 14th,21st and 28th day respectively. RT- PCR method was adopted to detect the infection of MHV- A59. The results showed that MHV were detected earliest in mice inoculated via belly, that is, 3 days in inbred strain mice an d immunodificient mice, and 7 days in transgenic mice.

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