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Position: Home > Articles > STUDY OF RT-PCR METHODS FOR MHV (MOUSE HEPATITIS VIRUS) DETERMINATION Journal of Southwest University(Natural Science Edition) 2004,26 (6) 762-763+772

小鼠肝炎病毒RT-PCR检测方法的研究

作  者:
赖国旗;何明忠;谭毅;王胜;潘永全;韦克;何伏秋;蒋虹;佟巍;丛喆
单  位:
中国医学科学院协和医科大学实验动物研究所;重庆医科大学实验动物中心
关键词:
小鼠肝炎病毒;RT-PCR
摘  要:
用4株小鼠肝炎病毒(MHV1,MHV3,A59,JHM)分别感染DBT细胞,收获病毒,提取病毒RNA。根据病毒基因的特异性保守序列设计引物,在最佳条件下进行RT-PCR,建立了RT-PCR检测方法,即两步法和一步法,分别扩增出600bp,375bp的特异性核酸片段,其中,一步法检测MHV更快速、简便。
译  名:
STUDY OF RT-PCR METHODS FOR MHV (MOUSE HEPATITIS VIRUS) DETERMINATION
作  者:
LAI Guo-qi~1, HE Ming-zhong~1, TAN Yi~1, WANG Sheng~1, PAN Yong-quan~1 WEI Ke~1, HE Fu-qiu~2, JIANG Hong~2, TONG Wei~2, CONG Zhe~2 (1. Laboratory Animal Center, Chongqing University of Medical Sciences, Chongqing 400016, China; 2. Institute of Laboratory Animal Science, CANS, PUYMC, Beijing 100021, China)
关键词:
mouse hepatitis virus (MHV); RT-PCR
摘  要:
Four different strains of mouse hepatitis virus (MHV), MHV 1, MHV 3, A 59 and JHM, were used to infect DBT cells. Viral RNA was extracted from the culture liquid. Two pairs of primers were designed according to the specific conservative gene sequence of MHV. RT-PCR was performed under the optimum conditions. Two RT-PCR procedures were established for MHV detection: one-step and two-step procedures, which amplified specific nucleic acid segments of 600 bp and 375 bp, respectively. Comparatively, the one-step RT-PCR was quicker, simpler and more convenient.

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