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当前位置: 首页 > 文章 > RT-LAMP技术检测菜豆荚斑驳病毒的研究 植物保护 2010,36 (6) 139-141+146
Position: Home > Articles > Detection of Bean pod mottle virus by RT-LAMP Plant Protection 2010,36 (6) 139-141+146

RT-LAMP技术检测菜豆荚斑驳病毒的研究

作  者：
闻伟刚;杨翠云;崔俊霞;张颖
单  位：
上海出入境检验检疫局;宁波出入境检验检疫局
关键词：
菜豆荚斑驳病毒;环介导等温扩增;检测
摘  要：
菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)是我国对外公布的检疫性有害生物,本研究采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP),建立了一种快速、灵敏和特异的BPMV检测方法。根据BPMV外壳蛋白编码基因上的8个位点,共设计了6条引物,通过RT-LAMP扩增得到特征性的瀑布状条带。特异性试验表明,引物对BPMV的检测具有良好的特异性;灵敏度试验显示RT-LAMP比RT-PCR灵敏度高1 000倍。该方法无需特殊的试剂和设备,只需在水浴锅中60℃等温扩增,整个检测周期约2~2.5 h,适合BPMV的快速、准确检测。
译  名：
Detection of Bean pod mottle virus by RT-LAMP
作  者：
Wen Weigang1,Yang Cuiyun2,Cui Junxia1,Zhang Ying1(1.Ningbo Entry-Exit Inspection and Quarantine Bureau,Ningbo 315012,China;2.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China)
关键词：
Bean pod mottle virus(BPMV);loop-mediated isothermal amplification(LAMP);detection
摘  要：
Bean pod mottle virus(BPMV) is a quarantine pest as approved by Chinese government.A rapid,sensitive and specific BPMV detection method using loop-mediated isothermal amplification(LAMP) was established in this study.Based on the sequence of BPMV coat protein coding gene,six primers were designed and the characteristic waterfall-like bands were obtained by RT-LAMP.Specificity tests showed that the primers were specific to BPMV;sensitivity test showed that RT-LAMP was 1000 times higher than RT-PCR.The method,without special reagents and equipments,was conducted with 60 ℃ isothermal amplification in a water bath and the entire testing cycle was about 2-2.5 h.It is suitable for rapid and accurate detection of BPMV.

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