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当前位置: 首页 > 文章 > 单管多重RT-PCR同时检测大豆种子中三种检疫性植物病毒 植物保护 2016,42 (5) 113-117
Position: Home > Articles > Simultaneous detection of three quarantine plant viruses from soybean seeds by multiplex RT-PCR in single PCR tube Plant Protection 2016,42 (5) 113-117

单管多重RT-PCR同时检测大豆种子中三种检疫性植物病毒

作  者：
易汪雪;宋绍祎;吴东妮;代欢欢;杨翠云;于翠
单  位：
中国科学院上海生命科学研究院;合肥师范学院;上海出入境检验检疫局动植物与食品检验检疫技术中心
关键词：
菜豆荚斑驳病毒;烟草环斑病毒;番茄环斑病毒;多重RT-PCR;同时检测
摘  要：
我国大豆年进口量持续增长,快速、准确地检测进境大豆种子中可能携带的病原物是防止检疫性有害生物入境传播和扩散的有效手段。菜豆荚斑驳病毒(BPMV)、烟草环斑病毒(TRSV)和番茄环斑病毒(ToRSV)均是被大豆种子携带的检疫性有害生物。本研究建立了在单一PCR管中同时检测这3种检疫性植物病毒及大豆内源基因Bd-30K的多重RT-PCR方法。研究结果表明,所建立的多重RT-PCR方法具有较好的特异性和灵敏度,检测含BPMV、TRSV和ToRSV RNA的最低浓度分别为0.45、0.0093和0.004ng/μL,从大豆种子中同时检测3种病毒的最低RNA浓度为0.58ng/μL。
译  名：
Simultaneous detection of three quarantine plant viruses from soybean seeds by multiplex RT-PCR in single PCR tube
作  者：
Yi Wangxue;Song Shaoyi;Wu Dongni;Dai Huanhuan;Yang Cuiyun;Yu Cui;Technical Center for Animal,Plant and Food Inspection and Quarantine,Shanghai Entry-Exit Inspection and Quarantine Bureau;Shanghai Information Center for Life Sciences,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences;Hefei Normal College;
关键词：
Bean pod mottle virus;;Tobacco ringspot virus;;Tomato ringspot virus;;multiplex RT-PCR;;simultaneous detection
摘  要：
The quantity of soybean seeds imported were continually increased in recent years.Rapid detection of seed-borne pathogens is essential to efficiently prevent pest invading and spreading.Bean pot mosaic virus(BPMV),Tobacco ringspot virus(TRSV)and Tomato ringspot virus(ToRSV)are the quarantine pest which could be carried by the soybean seeds.We developed the multiplex RT-PCR approach for simultaneous detection of BPMV,TRSV,ToRSV and one endogenous gene Bd 30 K in the single PCR tube from soybean seeds.The results indicated that good specificity and sensitivity for simultaneous detection were obtained.The limit detection of RNA with BPMV,TRSV and ToRSV were 0.45 ng/μL,0.009 3 ng/μL and0.004 ng/μL,respectively.The limit detection of RNA with three viruses mixture was 0.58 ng/μL.

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