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亚硒酸钠保护游离脂肪酸诱导肝细胞功能和Caspase3活性的变化研究

作  者:
张鹏春;陈丽君;范瑞威;郭苗苗;郭志侯;张志刚
单  位:
东北农业大学动物医学学院
关键词:
亚硒酸钠;游离脂肪酸;细胞增殖率;肝细胞功能;Caspase3
摘  要:
为研究亚硒酸钠(Na2SeO3)对游离脂肪酸(FFA)诱导大鼠肝细胞功能和Caspase 3活性变化的作保护用,采用胶原酶两步灌流法分离培养肝细胞,在细胞培养液中添加FFA和Na2SeO3(0.10μmol/L),测定肝细胞上清液生化指标、细胞增殖率和Caspase 3活性。结果表明,培养液中添加FFA,细胞增殖率降低,肝细胞功能受到影响,Caspase 3活性升高,且FFA的这种作用呈剂量依赖性;Na2SeO3能抑制由FFA引起的肝细胞的上述变化。
译  名:
The protective effects of sodium selenite on liver function and the activity of Caspase 3 induced by free fatty acids
作  者:
Zhang Pengchun,Chen Lijun,Fan Ruiwei,et al.
关键词:
sodium selenite;free fatty acids;cell proliferation rates;liver function;Caspase 3
摘  要:
The experiment was conducted to study the effect of sodium selenite on liver function and the activity of Caspase 3 induced by free fatty acids(FFA).Primary rat hepatocytes were obtained by two-step collagenase perfusion method.Cell proliferation rates,liver function and the activity of Caspase 3 in FFA-treated group with or without sodium selenite(0.10 μmol/L) were determined.The results showed that:FFA changed dose-dependently cell proliferation rates,liver function and the activity of Caspase 3,whereas the presence of sodium selenite reversed these alternations.

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