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当前位置: 首页 > 文章 > 磷脂酶参与二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用 生态学杂志 2019 (4) 995-1003
Position: Home > Articles > Phospholipase is involved in the allelopathy of diterpenoid oridonin to Arabidopsis thaliana Chinese Journal of Ecology 2019 (4) 995-1003

磷脂酶参与二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用

作  者：
安炎黄;赵启安;陈璐;赵敏;张莉环;杨宁
单  位：
西北师范大学生命科学学院
关键词：
冬凌草甲素;磷脂酶;化感作用;突变体
摘  要：
为探究二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用机制,本研究以冬凌草甲素为供体,以哥伦比亚野生型拟南芥WT和3个拟南芥磷脂酶A_1(PLA_1)、磷脂酶A_2(PLA_2)、磷脂酶C(PLC)的突变体株系pla_1、pla_2和plc_1为受体植物,通过施加不同浓度的冬凌草甲素(10、60μmol·L~(-1)),对4种拟南芥株系的种子萌发率、幼苗生长状况、相对电导率(EL)、丙二醛(MDA)含量以及3种磷脂酶活性进行了检测。结果表明:外施不同浓度的冬凌草甲素后,拟南芥种子萌发受到抑制,PLA_1和PLA_2参与此抑制过程;根长和下胚轴生长呈现典型的"低促高抑"趋势,PLA_1参与其对根的调控,PLC参与其对下胚轴的调控; 3种磷脂酶PLA_1、PLA_2和PLC酶活性均有不同程度的变化; EL和MDA增大且与浓度和时间呈正相关,PLA_2正调控10μmol·L~(-1)冬凌草甲素增大EL的过程,PLA_1和PLA_2共同响应60μmol·L~(-1)冬凌草甲素增大EL的过程; PLA_1和PLA_2共同作用正调控MDA积累过程;冬凌草甲素对植物的化感作用与磷脂酶有关,植物通过调控磷脂酶响应了冬凌草甲素的部分化感作用,且不同的磷脂酶在调控植物响应冬凌草甲素的生长过程中具有不同的作用。
译  名：
Phospholipase is involved in the allelopathy of diterpenoid oridonin to Arabidopsis thaliana
作  者：
AN Yan-huang;ZHAO Qi-an;CHEN Lu;ZHAO Min;ZHANG Li-huan;YANG Ning;College of Life Sciences,Northwest Normal University;
单  位：
AN Yan-huang%ZHAO Qi-an%CHEN Lu%ZHAO Min%ZHANG Li-huan%YANG Ning%College of Life Sciences,Northwest Normal University
关键词：
oridonin;;phospholipase;;allelopathy;;mutant
摘  要：
We investigated the allelopathy mechanism of the diterpenoid oridonin to Arabidopsis thaliana.The germination rate,seedling growth,electrolyte leakage(EL),malondialdehyde(MDA),and three phospholipase activities(PLA_1,PLA_2 and PLC) of four Arabidopsis thaliana lines(the Colombia ecotype WT,and three phospholipase deletion mutants pla_1,pla_2 and plc_1)were measured under different oridonin concentrations(10 and 60 μmol · L~(-1)).The results showed that germination was inhibited by oridonin,and PLA_1 and PLA_2 participated in this process.The growth of root and hypocotyl showed a "low-promotion and high-inhibition"effect.PLA_1 was involved in the regulation of roots.PLC was involved in the regulation of hypocotyl.The activities of different phospholipases changed greatly under different oridonin concentrations.The EL and MDA increased and were positively correlated with treatment concentration and duration.The stimulation of EL by 10 μmol·L~(-1) of oridonin was regulated by PLA_2.The stimulation of EL by 60 μmol·L~(-1) of oridonin was co-regulated by PLA_1 and PLA_2.PLA_1 and PLA_2 regulated the MDA accumulation under oridonin treatment.Our results showed that phospholipase involves in the allelopathy of diterpenoid oridonin to Arabidopsis thaliana.Different phospholipases have different roles in regulating the response of plant to oridonin during its growth.

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