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当前位置: 首页 > 文章 > 磷脂酶Dα1与H_2S参与冬凌草甲素对拟南芥的化感作用 应用生态学报 2020 (3) 959-968
Position: Home > Articles > Phospholipase Dα1 and hydrogen sulfide were involved in the allelopathy of oridonin to Arabidopsis thaliana Chinese Journal of Applied Ecology 2020 (3) 959-968

磷脂酶Dα1与H_2S参与冬凌草甲素对拟南芥的化感作用

作  者：
赵启安;刘博;张玥;刘琴;张莉环;刘瑞芳;杨宁
单  位：
西北师范大学生命科学学院;细胞活动与逆境适应教育部重点实验室
关键词：
冬凌草甲素;磷脂酶Dα1;硫化氢;化感作用
摘  要：
以拟南芥哥伦比亚野生型(WT)、磷脂酶Dα1(PLDα1)缺失型突变体pldα1、D-/L-半胱氨酸脱巯基酶(D-/L-CDes)缺失型突变体d-cdes和l-cdes幼苗为试验材料,60μmol·L~(-1)冬凌草甲素为处理浓度,研究了拟南芥响应二萜类化合物冬凌草甲素的化感作用中磷脂酶Dα1(PLDα1)与气体信号分子硫化氢(H_2S)的信号关系。结果表明:冬凌草甲素显著提高了野生型拟南芥幼苗H_2S含量、PLD和D-/L-CDes酶的活性及其基因表达;冬凌草甲素处理下,pldα1突变体幼苗的D-CDes和L-CDes活性明显低于WT,外源添加磷脂酸(PA)后D-CDes和L-CDes活性显著提高,并高于WT;冬凌草甲素显著抑制4种株系根的生长,其中d-cdes和l-cdes对冬凌草甲素更加敏感,外施NaHS可以促进冬凌草甲素处理下4种株系根的生长及内源H_2S产生,外施PA只对冬凌草甲素处理下的WT、pldα1和l-cdes株系根的生长及内源H_2S产生有促进作用,而对d-cdes株系没有明显作用。说明PLDα1和H_2S在拟南芥响应冬凌草甲素过程中发挥作用,且PLDα1/PA位于D-CDes上游,参与调控拟南芥幼苗H_2S的产生及根生长的信号过程。
译  名：
Phospholipase Dα1 and hydrogen sulfide were involved in the allelopathy of oridonin to Arabidopsis thaliana
作  者：
ZHAO Qi-an;LIU Bo;ZHANG Yue;LIU Qin;ZHANG Li-huan;LIU Rui-fang;YANG Ning;College of Life Sciences, Northwest Normal University;Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education;
关键词：
oridonin;;phospholipase Dα1;;hydrogen sulfide;;allelopathy
摘  要：
We investigated the signal relationship between phospholipase Dα1(PLDα1) and the gas signal molecule hydrogen sulfide(H_2S) in Arabidopsis thaliana response to the allelopathy of diterpenoid oridonin. The wild type Arabidopsis Columbia(WT), phospholipase Dα1(PLDα1) deletion mutant pldα1, D-/L-cysteine desulfyrase synthetic deletion mutant d-cdes and l-cdes seedlings were used as experiment materials, while 60 μmol·L~(-1) oridonin was applied as treatment concentration. The results showed that oridonin significantly increased H_2S content, PLD and D-/L-CDes activities, and gene expressions of PLDα1 and D-/L-CDes in WT. Under oridonin treatment, the D-CDes and L-CDes activities of pldɑ1 seedlings were significantly lower than those of WT. Both D-CDes and L-CDes activities increased after exogenous addition of phosphatidic acid(PA) and were higher than those of WT. Oridonin significantly inhibited root growth of four lines, with d-cdes and l-cdes being more sensitive to oridonin. Application of NaHS promoted root growth and endogenous H_2S production of four lines under oridonin treatment, while application of PA increased root growth and endogenous H_2S production in WT, pldɑ1 and l-cdes, but had no effect in d-cdes. These results indicated that PLDα1 and H_2S played a vital role in driving the response of Arabidopsis to oridonin, and that PLDα1/PA was located at the upstream of D-CDes to participate the regulation of the H_2S production and root growth.

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