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当前位置: 首页 > 文章 > 流加氨水提高木聚糖酶活力初步研究 河南农业科学 2007,36 (5) 29-31
Position: Home > Articles > A Preliminary Study on the Increasing Xylanase Activities by Feeding Ammonia Water Journal of Henan Agricultural Sciences 2007,36 (5) 29-31

流加氨水提高木聚糖酶活力初步研究

作  者：
鲁晓娟;吕帅;刘新育;康静;茹高林;陈红歌;贾新成
单  位：
河南农业大学生命科学学院
关键词：
黑曲霉;木聚糖酶;酸性蛋白酶;流加氨水
摘  要：
用正常产酶培养基和流加氨水产酶培养基培养Aspergillus niger J506,测定木聚糖酶的活性、酸性蛋白酶的活性和pH值。结果表明,用正常培养基培养时,木聚糖酶的活性在78~82 h达到产酶高峰,酶活力为226 U/mL。流加氨水培养,发酵液的pH值控制在4.0以上,木聚糖酶活力在86~90 h达到产酶高峰,活力为319 U/mL,比正常发酵提高了41.15%;而流加氨水条件下酸性蛋白酶活性从64 h开始比正常培养有明显降低。蛋白酶活力的降低和木聚糖酶活力的提高可能有一定关系。
译  名：
A Preliminary Study on the Increasing Xylanase Activities by Feeding Ammonia Water
作  者：
LU Xiao-juan,LU Shuai,LIU Xin-yu,KANG Jing,RU Gao-lin,CHEN Hong-ge*,JIA Xin-cheng (College of Life Science,Henan Agricultural University,Zhengzhou 450002,China)
关键词：
Aspergillus niger;Xylanase;Acidic protease;Feeding ammonia water
摘  要：
Aspergillus niger J506 was cultivated in both normal fermentation medium and medium feeding ammonia water,from which xylanase,protease and pH were measured.The results showed that the activity of xylanase in A.niger J506 reached the highest level of 226 U/ mL at 78-82 th hour in normal fermentation medium.By feeding ammonia water and keeping pH value above 4.0,the xylanase activity reached the highest level of 319 U/ mL at 86-90 th hour,41.15% higher than in normal fermentation medium.Meanwhile,the acidic protease activity had an obvious decrease after 64 hours by feeding ammonia water compared with that in normal fermentation medium,which probably had some relation to the higher level of xylanase.

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