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Position: Home > Articles > RP-HPLC Determination of Total Phenolic Compounds in Hawthorn FOOD SCIENCE 2009,30 (14) 163-166

山楂多酚含量的反相高效液相色谱法测定

作  者：
刘建利;原江锋;阎娥;李秀君;张志琪
单  位：
青海师范大学化学系;陕西师范大学化学与材料科学学院
关键词：
山楂;多酚;反相高效液相色谱
摘  要：
建立同时测定山楂中绿原酸、表儿茶素和芦丁3种酚类物质的反相高效液相色谱方法,并研究这三种酚类物质在山楂干果果肉、干果果核和鲜果果肉中的含量。经测定,表儿茶素在山楂鲜果果肉中含量最高,达到(1.661±0.024)mg/g;绿原酸和芦丁在山楂干果果肉中含量最高,分别达到(0.550±0.002)mg/g和(0.498±0.002)mg/g。本方法样品处理简单,具有良好的重现性和线性,相关性系数均达到0.999,绿原酸、表儿茶素和芦丁的回收率分别为91.8%～110.9%、94.6%～107.9%和94.2%～108.8%;检出限分别为0.14、0.37和0.68μg/ml(S/N=3)。
译  名：
RP-HPLC Determination of Total Phenolic Compounds in Hawthorn
作  者：
LIU Jian-li1,YUAN Jiang-feng1,YAN E2,LI Xiu-jun1,ZHANG Zhi-qi1,* (1. School of Chemistry and Material Science, Shaanxi Normal University, Xi'an 710062, China; 2. Department of Chemistry, Qinghai Normal University, Xining 810008, China)
关键词：
hawthorn;phenolic compounds;reversed-phase high performance liquid chromatography
摘  要：
A simple, rapid and sensitive reversed-phase high-performance liquid chromatography method (RP-HPLC) was developed for the simultaneous determination of chlorogenic acid, epicatechin and rutin in hawthorn. The contents of these phenolic compounds in pulp and stone of dry fruit and pulp of fresh fruit were determined. The pulp of fresh hawthorn fruit had a highest epicatechin content of (1.661 ± 0.024) mg/g and the contents of chlorogenic acid and rutin in pulp of dry hawthorn fruit both were the highest, reaching (0.550 ± 0.002) mg/g and (0.498 ± 0.002) mg/g, respectively. The regression equations for the three analytes presented good linearity and all the correlation coefficients were over 0.999. This method had good precision and repeatability. The spike recoveries for chlorogenic acid, epicatechin and rutin ranged from 91.8% to 110.9%, 94.6% to 107.9% and 94.2% to 108.8%, respectively, and the detection limits were 0.14, 0.37 and 0.68 μg/ml, separately.

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