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Position: Home > Articles > Identification of Eight Varieties of Bullbus Fritillaria cirrhosa Southwest China Journal of Agricultural Sciences 2014,27 (6) 2559-2563

八个川贝母品种的分类鉴定

作  者:
苏鹏;胡莉;董品利
单  位:
四川省茂县农业局;四川省农科院分析测试中心;四川省原子能研究院
关键词:
川贝母;鉴定;高效液相色谱-蒸发光散射检测器;trnH-psbA序列;ISSR分子标记
摘  要:
对8个川贝母品种采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)进行贝母碱分析、基于百合科植物特征序列trn H-psb A的分析、以及ISSR分子标记分析。结果表明,3种方法对川贝母的鉴定都是有效的。8个川贝母品种根据不同的贝母素含量均能独立区分开,trn H-psb A序列的分析和ISSR分子标记分析的聚类结果基本相符。
译  名:
Identification of Eight Varieties of Bullbus Fritillaria cirrhosa
作  者:
SU Peng;HU Li;DONG Pin-li;Sichuan Institute of Atomic Energy;Analysis and Testing Center,Sichuan Academy of Agricultural Sciences;Agricultural Bureau of Mao County;
关键词:
Bullbus Fritillaria Cirrhosa;;Identification;;HPLC-ELSD;;trn H-psb A sequence;;ISSR markers
摘  要:
By using HPLC-ELSD to conduct peimine analysis,sequence analysis of trn H-psb A based on Liliaceae Plants and ISSR molecular markers analysis,the results show that the three methods are quite effective to identity the eight varieties of bulbus fritillariae cirrhosae. They can be separated because of different peimine contents. Sequence analysis of trn H-psb A and cluster results of ISSR molecular markers are quite the same.

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