单 位：
天津农学院天津市畜牧兽医研究所;天津市畜牧兽医研究所
关键词：
抗菌肽;合生素;AA肉鸡;总蛋白;三酰甘油;谷丙转氨酶;谷胱甘肽过氧化物酶;肠道菌群
摘 要：
为了研究饲粮中添加抗菌肽、合生素对AA肉鸡血液生化指标和肠道菌群的影响,试验将480只1日龄AA肉鸡分为4组,1组为对照组,饲喂基础日粮;2组为添加抗菌肽组,饲喂基础日粮+0.5%抗菌肽;3组为添加合生素组,饲喂基础日粮+0.3%合生素;4组为添加抗菌肽+合生素组,饲喂基础日粮+0.5%抗菌肽+0.3%合生素,试验期为42 d。结果表明:添加抗菌肽显著提高了21日龄肉鸡的血清总蛋白、白蛋白、Ig G和Ig A水平(P<0.05);添加合生素显著提高了21日龄肉鸡血清谷胱甘肽过氧化物酶活性(P<0.05),降低了血清三酰甘油含量、谷丙转氨酶和谷草转氨酶活性(P<0.05)。添加抗菌肽显著提高了42日龄肉鸡血清总蛋白、白蛋白水平,降低了血清三酰甘油含量、谷丙转氨酶和谷草转氨酶活性(P<0.05);添加合生素显著提高了42日龄肉鸡的谷胱甘肽过氧化物酶活性并显著降低了谷丙转氨酶活性(P<0.05)。抗菌肽、合生素以及二者共同添加能显著或极显著降低肉鸡十二指肠、空肠、回肠和盲肠中大肠杆菌和沙门氏菌数,提高乳酸杆菌数(P<0.05或P<0.01)。说明抗菌肽、合生素单独或联合使用均能改善肉鸡的血清生化指标和肠道菌群状况,提高免疫力。
译 名：
Effects of antimicrobial peptides and synbiotics on serum biochemical indexes and intestinal microflora in AA broilers
作 者：
LIU Xiaolong;QIAO Jiayun;FAN Huan;MENG Fanrui;MA Qiang;WANG Wenjie;Tianjin Agricultural University;Tianjin Animal Husbandry and Veterinary Research Institute;
关键词：
antimicrobial peptide;;synbiotics;;AA broiler;;total protein;;triacylglycerol;;alanine aminotransferase;;glutathione peroxidase;;intestinal microflora
摘 要：
To investigate the effects of dietary antimicrobial peptides and synbiotics on blood biochemical indexes and intestinal microflora in AA broiler chickens,480 one-day-old AA broilers were randomly divided into 4 groups. Group 1 was fed basal diet as a control group,and group 2 was fed basal diet supplemented with 0. 5% antimicrobial peptides as the group added with antimicrobial peptides,and group 3 was fed basal diet supplemented with 0. 3% synbiotics as the group added with synbiotics,and group 4 was fed basal diet supplemented with 0. 5% antimicrobial peptides and 0. 3% synbiotics as the group added with antimicrobial peptides and synbiotics. The test lasted for 42 d. The results showed that adding antimicrobial peptides significantly increased( P < 0. 05) the levels of total protein,albumin,immunoglobulin G( Ig G) and immunoglobulin A( Ig A) in serum of 21-day-old broilers,while adding synbiotics significantly increased( P < 0. 05) the activity of glutathione peroxidase and significantly decreased( P < 0. 05) the content of serum triacylglycerol and the activities of alanine aminotransferase and aspartate transaminase in serum of 21-day-old broilers; adding antimicrobial peptides significantly increased( P < 0. 05) the levels of total protein,albumin and significantly decreased( P < 0. 05) the content of serum triacylglycerol and the activities of alanine aminotransferase and aspartate transaminase in serum of 42-day-old broilers,while adding synbiotics significantly increased( P < 0. 05) the activity of glutathione peroxidase and significantly decreased( P < 0. 05) the activity of alanine aminotransferase in serum of 42-day-old broilers. The numbers of Escherichia coli and Salmonella in the duodenum,jejunum,ileum and cecum of AA broilers were significantly or extremely significantly decreased( P < 0. 05 or P < 0. 01) and the number of Lactobacilli was significantly or extremely significantly increased( P < 0. 05 or P < 0. 01) after adding antimicrobial peptides and synbiotics alone or in combination. The results indicate that antimicrobial peptides and synbiotics used alone or in combination can improve the serum biochemical indexes and the condition of intestinal microflora in broilers,enhancing immunity.
