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当前位置: 首页 > 文章 > 牦牛线粒体DNA细胞色素b基因序列测定及其起源、分类地位研究 畜牧兽医学报 2006,37 (11) 1118-1123
Position: Home > Articles > Sequencing Cytochrome b Gene of Mitochondrial DNA in Yak and Researching Its Origin and Taxonomic Status Chinese Journal of Animal and Veterinary Sciences 2006,37 (11) 1118-1123

牦牛线粒体DNA细胞色素b基因序列测定及其起源、分类地位研究

作  者：
李齐发;李隐侠;赵兴波;刘振山;张庆波;宋大伟;屈旭光;李宁;谢庄
单  位：
南京农业大学动物科技学院;中国农业大学动物科技学院
关键词：
牛亚科;牦牛;线粒体;细胞色素b基因;起源;分类地位
摘  要：
牦牛在牛亚科中的分类地位仍存在较大的分歧。根据普通牛线粒体基因组序列设计引物对家牦牛基因组进行PCR扩增和克隆测序,获得了家牦牛细胞色素b(Cytochrome b)基因的全长序列,并以羊亚科绵羊(Ovis aries)为外类群,对牛亚科代表性物种进行了系统发育分析。结果显示:牛亚科不同物种间线粒体细胞色素b基因的转换/颠换比值为4.9,突变未达到饱和状态;牦牛与牛属间的序列差异百分比为8.0%~8.6%,大于牦牛与美洲野牛间的序列差异百分比;系统发育分析发现家牦牛与野牦牛首先聚为一类,再与美洲野牛聚为一类;说明牦牛与美洲牦牛属间的遗传相似性较高,而与牛属间的遗传相似性较低,结果支持现在的家牦牛和野牦牛都是同一祖先———原始牦牛的后代,推测两者分化时间大约为0.55百万年前;支持将牦牛划分为牛亚科牦牛属的观点,牦牛属包括家牦牛和野牦牛两个种。
译  名：
Sequencing Cytochrome b Gene of Mitochondrial DNA in Yak and Researching Its Origin and Taxonomic Status
作  者：
LI Qi-fa~1,LI Yin-xia~2,ZHAO Xing-bo~(2,3),LIU Zhen-shan~1,ZHANG Qing-bo~1,SONG Da-wei~1,QU Xu-guang~1,LI Ning~(2,3),XIE Zhuang~(1*)(1.College of Animal Science and Technology,Nanjing Agricultural University,Nanjng 210095,China;2.College of Animal Science and Technology,China Agricultural University,Beijng 100094,China;3.State Key Laboratory for Agribiotechnology,China Agricultural University,Beijing 100094,China)
关键词：
Bovinae;yak;mitochondria;Cytochrome b gene;origin;taxonomic status
摘  要：
There still exists remarkable difference on yak's taxonomic status in Bovinae.Primers designed according to the Mitochondrial gene sequences of Bos taurus reported was used to amplify and sequence yak's Cytochrome b gene,and the whole sequence of Cytochrome b gene was obtained.Using Ovis aries as outgroup taxa,the Phylogeny about the representative species of Bovinae was analyzed.The results showed that among different species the transition/transversion(Ts/Tv) of Cytochrome b gene was 4.9,suggesting that the mutation was not saturation;the percentage nucleotide sequence divergence between yak and Bovinae was 8.0%~8.6%,which was more than that of yak and Bison bison;Phylogeny analysis found that Poephagus grunniens and Poephagus mutus clustered first of all,then gathered with Bison bison,indicating there was higher genetic comparability than that of Bos,the results sustained the idea Poephagus grunniens and Poephagus mutus owned one ancestor —primitive yak;The approximate divergence time between these two species was 0.55 million years.The data also supports the point that yak is classified as Poephagus of Bovinae,Poephagus including two species: Poephagus grunniens and Poephagus mutus.

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