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当前位置: 首页 > 文章 > 双波长比色法测定糜子中直链/支链淀粉含量 中国农学通报 2015,31 (32) 76-80
Position: Home > Articles > Determination of Amylose /Amylopectin in Panicum miliaceum L. by Dual-Wavelength Colorimetric Method Chinese Agricultural Science Bulletin 2015,31 (32) 76-80

双波长比色法测定糜子中直链/支链淀粉含量

作  者：
田翔;乔治军;陈艳霞
单  位：
山西省农业科学院农作物品种资源研究所农业部黄土高原作物基因与种质创制重点实验室杂粮种质资源发掘与遗传改良山西省重点实验室
关键词：
双波长法;糜子;直链淀粉;支链淀粉
摘  要：
为建立一种同时测定糜子中直链和支链淀粉含量的简便方法,充实糜子理化性质的相关研究,首先应用双波长比色法,结合糜子直链淀粉/支链淀粉—碘复合物的吸收光谱图,确定其测定波长与参比波长。并利用标准工作曲线,求出样品中直链/支链淀粉的含量。由全波长扫描图谱确定了糜子中直链淀粉测定波长与参比波长分别为613 nm与520 nm,支链淀粉的测定波长和参比波长分别为538 nm与700 nm。结果表明,直链淀粉与支链淀粉的浓度分别在0~0.15 mg/m L和0~0.125 mg/m L呈线性相关。实验室用此法对15份糜子种质中直链与支链淀粉的含量进行测定。通过精密度与回收率实验,测得直链与支链淀粉的6次的标准偏差分别为0.145与0.022,回收率范围85.3%~97.9%。试验结果均满足分析要求,所建立的方法具有简便、快速、准确等特点,可用于测定糜子中直链和支链淀粉的含量。可对糜子口感及品质进行评价,从而筛选出优良种质。
译  名：
Determination of Amylose /Amylopectin in Panicum miliaceum L. by Dual-Wavelength Colorimetric Method
作  者：
Tian Xiang;Qiao Zhijun;Chen Yanxia;Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Gene Resources & Germplasm Enhancement on Loess Plateau, Ministry of Agriculture,Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops;
关键词：
dual-wavelength colorimetric method;;Panicum miliaceum L.;;amylose;;amylopectin
摘  要：
In order to establish a new and simple determination method of amylose and amylopectin in Panicum miliaceum L. and enrich its related research on physical and chemical properties, the paper useddual- wavelength colorimetric method and the absorption spectra of amylose/amylopectin- I2 complexes todetermine the measuring wavelength and reference wavelength firstly. The contents of amylose and amylopectinin sample solution were determined according to the linear regression equations. Measure wavelength andreference wavelength of amylose determined by the wavelength scanning graph were 613 nm and 520 nm,respectively. Measure wavelength and reference wavelength of amylopectin were 538 nm and 700 nm,respectively. The result showed that linear relationship of amylose and amylopectin concentrations were in therange of 0-0.15 mg/m L and 0-0.125 mg/m L. The amylase and amylopectin contents of 15 kinds of Panicum miliaceum L. were measured using the method in lab. Through the precision and recovery experiment, amyloseand amylopectin were measured 6 times and the standard deviation was 0.145 and 0.022, respectively, therecovery range was 85.3%-97.9%. Test results met the analysis requirement. Dual-wavelength method was simple, rapid and accurate and was suitable to determine amylose and amylopectin in Panicum miliaceum L..The method could evaluate Panicum miliaceum L. taste and quality, and then screen palatability of excellentvariety.

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