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Position: Home > Articles > Extraction,Purification and Characterization of Morin from Xinjiang Black Mulberry(Morus nigra L.)Tree Branch FOOD SCIENCE 2011,32 (2) 76-78

新疆药桑桑枝中桑色素的提取分离及鉴定

作  者:
周林;徐立;余茂德;刘峻池;黄先智
单  位:
西南大学生物技术学院
关键词:
新疆药桑;桑色素;桑枝;提取分离;鉴定
摘  要:
研究新疆药桑桑枝中桑色素的分离纯化工艺。采用乙醇浸提,有机溶剂萃取,聚酰胺树脂吸附色谱法对桑色素进行分离纯化;薄层层析色谱与高效液相色谱相结合的方式进行鉴定和检测。结果表明,经乙酸乙酯萃取,聚酰胺分离两次可纯化出桑色素;此外,在波长370nm处桑色素具有良好的色谱吸收峰。聚酰胺能分离得到较高纯度的桑色素,是分离黄酮类化合物较好的材料。
译  名:
Extraction,Purification and Characterization of Morin from Xinjiang Black Mulberry(Morus nigra L.)Tree Branch
作  者:
ZHOU Lin,XU Li*,YU Mao-de,LIU Jun-chi,HUANG Xian-zhi(College of Biotechnology,Southwest University,Chongqing 400716,China)
关键词:
Morus nigra L.;morin;mulberry tree branch;extraction and purification;identification
摘  要:
A process for the preparation of morin from Xinjiang black mulberry(Morus nigra L.)tree branch using organic solvent extraction and polyamide resin column chromatographic purification is presented in this paper.Thin layer chromatography and HPLC were integratively used for the qualitative analysis of morin.The 95%ethanol extract from black mulberry tree branch was reextracted separately with ethyl acetate and chloroform,and the thin layer chromatographic results showed that morin was present in the ethyl acetate phase rather than the chloroform phase.High-purity morin was obtained after twice purification on polyamide resin column,which displayed excellent characteristic adsorption at 370 nm wavelength.Thus,polyamide resin is a promising material for the purification of flavonoid,like morin.

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