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当前位置: 首页 > 文章 > 新疆药桑枝条醇提物不同溶剂萃取组分的抗氧化活性物质含量及抗氧化活性测试 蚕业科学 2013,v.39 (5) 978-983
Position: Home > Articles > Contents and Antioxidant Activity of Antioxidant Substances in Various Organic Solvent Extraction Fractions from Xinjiang Medicinal Mulberry Branch Ethanolic Extract Science of Sericulture 2013,v.39 (5) 978-983

新疆药桑枝条醇提物不同溶剂萃取组分的抗氧化活性物质含量及抗氧化活性测试

作  者：
刘静;刘超;王传宏;徐立;向伟;明月;吴丽莉;丁天龙
单  位：
西南大学生物技术学院;新疆维吾尔自治区和田蚕桑科学研究所
关键词：
新疆药桑;桑枝醇提物;萃取组分;石油醚;乙酸乙酯;总多酚;总黄酮;1,1-二苯基苦基苯肼
摘  要：
以新疆药桑桑枝醇提物及其不同有机溶剂萃取组分为供试样品,采用薄层色谱(TLC)-自显影技术辅助检测分析样品的总多酚、总黄酮含量及抗氧化活性。桑枝醇提物中的石油醚萃取组分得率为2.85%,乙酸乙酯萃取组分得率为27.81%,正丁醇萃取组分得率为36.63%,剩余水相组分得率为31.02%。石油醚萃取组分与乙酸乙酯萃取组分的抗氧化活性显著高于正丁醇萃取组分和剩余水相组分,前2种组分中的总多酚、总黄酮质量比之和分别为211.75 mg/g、764.27 mg/g。体外生物活性测试桑枝醇提物及石油醚萃取组分和乙酸乙酯萃取组分的还原力(以Vc含量计)分别为58.25、15.63、202.50 mg/g,对1,1-二苯基苦基苯肼(DPPH)自由基的半数抑制浓度(IC50)分别为0.52、0.32和0.05 g/L。测试结果提示:新疆药桑桑枝醇提物具有较强的抗氧化活性,且抗氧化活性物质主要存在于石油醚和乙酸乙酯萃取组分。
译  名：
Contents and Antioxidant Activity of Antioxidant Substances in Various Organic Solvent Extraction Fractions from Xinjiang Medicinal Mulberry Branch Ethanolic Extract
作  者：
LIU Jing;LIU Chao;WANG Chuan-Hong;XU Li;XIANG Wei;MING Yue;WU Li-Li;DING Tian-Long;College of Biotechnology,Southwest University;Hetian Sericultural Research Institute of Xinjiang Uighur Autonomous Region;
关键词：
Xinjiang medicinal mulberry;;Mulberry branch ethanolic extract;;Extraction fraction;;Petroleum ether;;Ethyl acetate;;Total polyphenolics;;Total flavonoids;;1,1-Diphenyl-2-picrylhydrazyl
摘  要：
In this study,Xinjiang medicinal mulberry( Morus nigra L.) branch ethanolic extract and its different organic solvent extraction fractions were used as test samples to detect and analyze the contents of total polyphenolics,total flavonoids and antioxidant activities using TLC-autography technology. The yield of petroleum ether extraction fraction,ethyl acetate extraction fraction,n-butanol extraction fraction,and residue water phase fraction was 2. 85%,27. 81%,36. 63%and 31. 02%,respectively. The antioxidant activities of petroleum ether and ethyl acetate extraction fractions were significantly higher than those of n-butanol extraction fraction and residue water phase fraction. The sum of mass ratios of total polyphenolics and total flavonoids in the first two extraction fractions was 211. 75 mg /g and 764. 27 mg /g. In vitro bioactivity assay displayed that the reducing power( calculated as equivalence to vitamin C) of mul- berry branch ethanolic extract,petroleum ether and ethyl acetate extraction fractions was 58. 25,15. 63 and 202. 50 mg /g respectively,and their inhibitory concentration 50%( IC50)in scavenging 1,1-diphenyl-2-picrylhydrazyl( DPPH) free radical was 0. 52,0. 32 and 0. 05 g /L respectively. The results demonstrated that Morus nigra L. branch ethanolic extract has strong antioxidant activity and the active substances mainly exist in petroleum ether and ethyl acetate extraction fractions.

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