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Position: Home > Articles > Process Optimization for Total Polyphenol Extraction from the Tree Branch Bark of Xinjiang Black Mulberry(Morus nigra L.)by Response Surface Methodology FOOD SCIENCE 2011,32 (2) 104-107

响应曲面法优化新疆药桑桑皮总多酚提取工艺

作  者:
伍春;徐立;刘峻池;黄先智;买买提依明
单  位:
新疆和田蚕桑科学研究所;西南大学生物技术学院
关键词:
新疆药桑;桑皮;总多酚;响应曲面
摘  要:
采用响应曲面法对新疆药桑桑皮中多酚类化合物的提取工艺进行优化。在单因素试验的基础上,选择乙醇体积分数、提取温度、提取时间,进行三因素三水平的Box-Behnken中心组合试验设计,采用响应曲面法(RSM)分析3个因素对响应值的影响。结果表明:最佳提取工艺条件为溶剂中乙醇体积分数78%、提取温度70℃、提取时间240min、液料比30:1(mL/g),在此条件下,总多酚的得率达到0.793%。该工艺稳定可靠,可在生产中应用。
译  名:
Process Optimization for Total Polyphenol Extraction from the Tree Branch Bark of Xinjiang Black Mulberry(Morus nigra L.)by Response Surface Methodology
作  者:
WU Chun1,XU Li1,*,LIU Jun-chi1,HUANG Xian-zhi1,MAIMAITI Yi-ming2(1.College of Biotechnology,Southwest University,Chongqing 400716,China;2.Sericulture Research Institute of Hetian Xinjiang,Hetian 848000,China)
关键词:
Morus nigra L.;bark mulberry tree bark;total polyphenols;response surface methodology
摘  要:
The objective of this study was the optimization of the extraction process for total polyphenols from the tree branch bark of Xinjiang black mulberry(Morus nigra L.)by response surface methodology.Based on single factor experiments,a three-factor,three-level Box-Behnken central composite design(CCD)coupled with response surface analysis(RSM)was employed to probe the effects of ethanol concentration,extraction temperature and length of extraction time on the yield of total polyphenols.It was found that the best extraction conditions were as follows:ethanol concentration,78%;and liquid/material ratio,30:1(mL/g)for an extraction duration of 240 min at 70℃,and that a maximum total polyphenol yield of 0.793%under such conditions.The extraction process proves stable and reliable and is applicable for the production of polyphenols.

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