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当前位置: 首页 > 文章 > 高效液相色谱-串联质谱法测定猪血中盐酸可乐定和盐酸赛庚啶 肉类研究 2015 (6) 25-28
Position: Home > Articles > Determination of Clonidine Hydrochloride and Cyproheptadine Hydrochloride in Swine Blood by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry MEAT RESEARCH 2015 (6) 25-28

高效液相色谱-串联质谱法测定猪血中盐酸可乐定和盐酸赛庚啶

作  者：
杨挺;许秀琴;赵健;章豪;吴银良;王立君;朱勇
单  位：
宁波市农业科学研究院
关键词：
猪血;高效液相色谱-串联质谱;盐酸可乐定;盐酸赛赓啶
摘  要：
建立高效液相色谱-串联质谱法测定猪血中盐酸可乐定和盐酸赛庚啶的方法。样品中的盐酸可乐定和盐酸赛庚啶用乙腈提取,高效液相色谱-串联质谱法进行分析。分析时采用Waters BEH C18色谱柱(100mm×2.1mm,1.7μm),以0.10%甲酸水-甲醇作为流动相进行梯度洗脱,电喷雾正电子(ESI+)模式电离,多反应监测模式检测,外标法进行定量。结果表明:盐酸可乐定和盐酸赛庚啶标准溶液在1.0~50μg/L范围内,峰面积与含量呈线性相关。盐酸可乐定和盐酸赛庚啶的检出限均为0.20μg/kg,样品中添标回收率在85%~110%之间,相对标准偏差小于10%。
译  名：
Determination of Clonidine Hydrochloride and Cyproheptadine Hydrochloride in Swine Blood by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
作  者：
YANG Ting;XU Xiuqin;ZHAO Jian;ZHANG Hao;WU Yinliang;WANG Lijun;ZHU Yong;Ningbo Academy of Agricultural Sciences;
关键词：
swine blood;;ultra performance liquid chromatography-tandem mass spectrometry;;clonidine hydrochloride;;cyproheptadine hydrochloride
摘  要：
A simple and sensitive ultra performance liquid chromatography-tandem mass (UPLC-MS/MS) method for the determination of clonidine hydrochloride and cyproheptadine hydrochloride in swine blood was developed. Samples were extracted with acetonitrile and analyzed by UPLC-MS/MS on a Waters Acquity BEH C18 column with 0.10% formic acid in water/methanol as the mobile phase by means of gradient elution. Detection was carried out with an electrospray ionisation (ESI) probe and operated in the positive ion mode. Clonidine hydrochloride and cyproheptadine hydrochloride was quantified by the external standard method. There was a good linearity in the range of concentrations between 1.0 and 50.0 μg/L. The limits of detection for clonidine hydrochloride and cyproheptadine hydrochloride were both 0.2 μg/L. The recoveries from spiked samples were in the range of 85% to 110% and the relative standard deviations (RSD) were lower than 10%.

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