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Position: Home > Articles > Ultrasound-Assisted Enzymatic Hydrolysis of Porcine Blood to Prepare Antioxidant Peptides MEAT RESEARCH 2015 (11) 10-14

超声波辅助酶解制备猪血源抗氧化肽

作  者:
唐雯倩;成晓瑜;刘文营;刘成国
单  位:
湖南农业大学食品科学技术学院;中国肉类食品综合研究中心
关键词:
超声波;胰蛋白酶;猪血;抗氧化肽;1,1-二苯基-2-三硝基苯肼
摘  要:
超声波处理可以提高胰蛋白酶溶液的酶活力和溶解度。实验利用超声波处理胰蛋白酶溶液,其酶活力和蛋白酶溶解度分别提高104.9%和2.4%。针对胰蛋白酶水解猪血血红蛋白酶解液,通过单因素试验和正交试验优化,以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl?radical,DPPH)自由基清除率和胰蛋白酶水解度为指标,得到最佳抗氧化肽工艺优化条件为:超声频率20?k Hz、超声时间8?min、超声功率540 W、胰蛋白酶添加量0.8%、反应体系p H 8.0,酶解2 h即可达到常规酶解6 h的酶解度,此时DPPH自由基清除率为75.78%。应用超声波辅助处理可以提高酶解效率,缩短工作时间。
译  名:
Ultrasound-Assisted Enzymatic Hydrolysis of Porcine Blood to Prepare Antioxidant Peptides
作  者:
TANG Wenqian;CHENG Xiaoyu;LIU Wenying;LIU Chengguo;College of Food Science and Technology, Hunan Agricultural University;China Meat Research Center;
关键词:
ultrasound;;trypsin;;porcine blood;;antioxidant peptides;;1,1-dipheny1-2-picrylhydrazyl radical(DPPH)
摘  要:
The activity and solubility of trypsin were found to be increased by 104.9%and 2.4%after ultrasonic treatment,respectively.Based on this result,we proposed an ultrasound-assisted enzymatic hydrolysis process for preparing antioxidant peptides from porcine blood.The optimal hydrolysis parameters that produced the same degree of hydrolysis as that obtained after 6 h hydrolysis by the routine procedure were determined by using single-factor and orthogonal array designs based on1,1-diphenyl-2-picrylhydrazyl radical(DPPH) scavenging activity as follows:ultrasonic frequency,20 kHz;ultrasonic time,8 min;ultrasonic power,540 W;addition of trypsin,0.8%;and initial pH,8.0.The resulting hydrolysate could scavenge75.78%of DPPH radicals.To conclude,this study demonstrates that ultrasound treatment can increase the hydrolysis efficiency of porcine blood and shorten the hydrolysis time.

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