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Position: Home > Articles > The mRNA expression of MEF2A genes in different tissues of Xingyi duck Animal Husbandry & Veterinary Medicine 2017 (7) 42-45

兴义鸭MEF2A基因mRNA在组织中表达研究

作  者:
王成栋;李辉;卜小雁;师新彩;彭邦星;易恒洁;赵忠海
单  位:
贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室;贵州省遵义市正安县畜牧产业发展办公室;贵州省安顺市畜牧技术推广站;贵州省毕节市农业产业办公室
关键词:
MEF2A基因;组织表达;兴义鸭
摘  要:
运用RT-PCR和qRT-PCR对兴义鸭NEF2A基因在不同组织中的表达量进行探究。结果表明,心、肝、肺、肾、十二指肠、大脑、胸肌、腿肌、肌胃、腺胃共10个组织中均检测到了MEF2A基因mRNA的表达,说明该基因属于广谱表达基因;同时,还发现该基因呈依赖性组织表达,大脑中的表达量最高,消化器官中表达量相对最低,如肌胃和腺胃。研究结果为兴义鸭组织表达谱构建奠定了基础。
译  名:
The mRNA expression of MEF2A genes in different tissues of Xingyi duck
作  者:
WANG Chengdong;LI Hui;BU Xiaoyan;SHI Xincai;PENG Bangxing;YI Hengjie;ZHAO Zhonghai;College of Animal Sciences,Guizhou University,Key Laboratory of Genetic Breeding and Reproduction of Mountain Animals of Ministry of Education;Bijie Agricultural Industry Office of Guizhou Province;Anshun Station of Animal Husbandry Technology Extension of Guizhou Province;Developmental Office of Animal Husbandry Industry in Zhengan County of Guizhou Province;
关键词:
MEF2A gene;;tissues expression;;Xingyi duck
摘  要:
The aim of this study was to explore the expression of the NEF2 A gene in different tissues in Xingyi duck by RT-PCR and qRTPCR techniques. The results showed that the NEF2 A gene was a broad spectrum of expressed genes,because the expression of MEF2 A gene mRNA was detected in the 10 tissues in the experiment,including heart,liver,lung,kidney,duodenal,brain,chest,leg muscle,muscle stomach and glandular stomach. The expression pattern of the MEF2 A gene was tissue-dependent. Therefore,these results provide the basis for the construction of tissue expression profiles in Xingyi ducks.

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