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当前位置: 首页 > 文章 > 贵紫麦1号转录组EST-SSR标记发掘与应用 河南农业科学 2020,49 (5) 15-30
Position: Home > Articles > Exploration and Application of EST-SSR Markers in Guizimai No.1 Transcriptome Journal of Henan Agricultural Sciences 2020,49 (5) 15-30

贵紫麦1号转录组EST-SSR标记发掘与应用

作  者：
杨帅;杨喜翠;何方;李鲁华;李振华;任明见;徐如宏
单  位：
贵州大学农学院
关键词：
紫色小麦;EST-SSR;多态性筛选;标记开发;染色体定位;遗传多样性
摘  要：
为了开发新的小麦分子标记,利用前期小麦品种贵紫麦1号的转录组测序结果,对所得序列进行EST-SSR(Expressed sequence tags-simple sequence repeat)标记开发,并对开发的标记进行了染色体定位、多态性筛选。结果表明,在119 572条总Unigenes序列中得到8 749条含SSR位点的EST序列,占7.3%,平均每8.04 kb就会出现1个SSR;EST序列中SSR类型的分布特点为三核苷酸重复类型最多,而五、六核苷酸重复类型出现频次很少,单核苷酸重复以A/T出现频次最多,二核苷酸重复以AG/CT出现频次最多,三核苷酸重复以CCG/CGG出现频次最多;对含SSR位点的EST序列进行引物设计,成功设计出引物的序列有5 503条,占总EST序列的62.9%,从中筛选到341对有效性EST-SSR引物;以中国春为对照,利用16份中国春缺体-四体系材料对341对EST-SSR引物进行染色体定位,共定位153对引物、201个位点,涉及除3A、5A、3B、4B及1D外的16条染色体,其中5B染色体定位引物对最多,达23对;筛选出53对多态性EST-SSR引物,对52份小麦材料进行遗传多样性分析发现,其中18对EST-SSR引物可将52份小麦材料一一区分。
译  名：
Exploration and Application of EST-SSR Markers in Guizimai No.1 Transcriptome
作  者：
YANG Shuai;YANG Xicui;HE Fang;LI Luhua;LI Zhenhua;REN Mingjian;XU Ruhong;Agricultural College,Guizhou University;Guizhou Sub-center of National Wheat Improvement Center;
关键词：
Purple wheat;;EST-SSR;;Polymorphism screening;;Marker exploration;;Chromosome location;;Genetic diversity
摘  要：
In order to develop new wheat molecular markers,EST-SSR markers were developed based on the results of transcriptome sequencing of Guizimai No.1.The chromosome location,polymorphism of developed markers were studied.The results showed that 8 749 EST sequences containing SSR loci were obtained from 119 572 total unigenes sequences,accounting for 7.3%,with an average of 1 SSR per 8.04 kb.The distribution characteristic of SSR types in EST sequences was that the trinucleotide repeat was the most,and pentanucleotide and hexanucleotide repeats were less;A/T repeat was the most in the single nucleotide repeats,AG/CT repeat was the most in the dinucleotide repeats,and CCG/CGG repeat was the most in trinucleotide repeats.Primers were successfully designed for 5 503 EST sequences containing SSR loci,accounting for 62.9% of the total EST sequences;341 effective EST-SSR primer pairs were screened;341 EST-SSR primer pairs were located using 16 Chinese Spring nulli-tetrasome system materials,and 153 EST-SSR primer pairs were located in 201 loci,involving 16 chromosomes except 3 A,5 A,3 B,4 B and 1 D,of which primer pairs were the most on 5 B chromosome,up to 23 primer pairs; 53 polymorphic EST-SSR primer pairs were screened out,and genetic diversity analysis of 52 wheat germplasms showed that 18 EST-SSR primer pairs could distinguish 52 wheat germplasms one by one.

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