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Position: Home > Articles > Alpha-glucosidase Inhibitory Activity of EGCG in Caco-2 Cells Hubei Agricultural Sciences 2017 (13) 2479-2481+2601

儿茶素EGCG对Caco-2细胞α-葡萄糖苷酶活性的抑制作用研究

作  者:
刘淑媛;艾仄宜;陈玉琼;倪德江
单  位:
华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室
关键词:
茶;EGCG;蔗糖酶;麦芽糖酶;Caco-2细胞
摘  要:
试验对Caco-2细胞膜上蔗糖酶和麦芽糖酶这两种α-葡萄糖苷酶活性进行不同培养时间检测,并以儿茶素单体EGCG作为抑制剂,探究其对Caco-2来源的蔗糖酶和麦芽糖酶的抑制效果。结果表明,Caco-2细胞培养18 d后膜表面具有较高活性的蔗糖酶和麦芽糖酶,可用于进行α-葡萄糖苷酶抑制剂筛选。儿茶素EGCG具有蔗糖酶和麦芽糖酶抑制活性,抑制IC50分别为31.08μg/m L和36.44μg/m L。
译  名:
Alpha-glucosidase Inhibitory Activity of EGCG in Caco-2 Cells
作  者:
LIU Shu-yuan;AI Ze-yi;CHEN Yu-qiong;NI De-jiang;Key Laboratory of Horticultural Plant Biology, Ministry of Education/College of Horticulture and Forestry Sciences ,Huazhong Agricultural University;
关键词:
tea;;EGCG;;sucrase;;maltase;;Caco-2
摘  要:
This research used the Caco-2 cell monolayer model in vitro to simulate the α-glucosidase activity at different culture days. EGCG was used as an inhibitor to investigate the inhibition activities on sucrase and maltase in Caco-2 cells.The results illustrated that Caco-2 could be used to estimate α-glucosidase inhibitory activity after 18 days with high sucrase and maltase activities. EGCG showed inhibitory effect on sucrase and maltase in Caco-2 cells, with the IC50 values of 31.08μg/m L and 36.44 μg/m L, respectively.

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