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当前位置: 首页 > 文章 > 基于色谱分析的葛根异黄酮快速检测方法 江苏大学学报(自然科学版) 2013,34 (4) 399-404
Position: Home > Articles > Method of isoflavones quick detecting in pueraria root based on chromatography Journal of Jiangsu University (Natural Science Edition) 2013,34 (4) 399-404

基于色谱分析的葛根异黄酮快速检测方法

作  者：
刘杏荣;董英;姜松;朱道辰
单  位：
江苏大学
关键词：
葛根异黄酮;色谱分析;薄层层析法;高效液相色谱法;胶束毛细管法
摘  要：
研究了分析葛根中葛根素和大豆苷含量色谱法的各种条件,包括TLC中展开剂与显色方法的选择、HPLC色谱分离条件及其检测特点、MEKC色谱分离条件及其检测特点等,优选出TLC,HPLC和MEKC的最佳色谱分离条件,并与葛根药典检测方法进行比较.结果表明:MEKC检测葛根素的准确性和精密度比HPLC略低,对大豆苷的灵敏度较差,但由于其具有机溶剂消耗小、出峰时间更短、与环境友好的突出特点,值得进一步探讨;TLC能快速鉴别葛根素、大豆苷等异黄酮物质;HPLC中葛根素和大豆苷的检测限低、检测范围宽,且稳定性、准确性和精密度较高.基于优化条件下的TLC-HPLC法能实现对葛根中葛根素和大豆苷的定性和定量快速准确检测.
译  名：
Method of isoflavones quick detecting in pueraria root based on chromatography
作  者：
Liu Xingrong~1,Dong Ying~1,Jiang Song~1,Zhu Daochen~2 (1.School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China;2.School of Environment,Jiangsu University,Zhenjiang,Jiangsu 212013,China)
关键词：
pueraria isoflavones;;chromatography;;TLC;;HPLC;;MEKC
摘  要：
The optimization chromatography analyses of puerarin and daidzin content were investigated under various conditions of the selections for TLC solvent and coloration,HPLC chromatographic separation conditions and detection characteristics,and MEKC chromatographic separation conditions and detection characteristics.The results show that the correctness and precision of MEKC are slightly lower than those of HPLC with lower sensitivity of daidzin,while it is worth further studying due to the low organic solvent consumption, quick peak flowing out and environmental friendly characteristics.Isoflavones of puerarin and daidzin can be rapidly identified by TLC with better separation efficiency than that in developing solvent of pharmacopoeia method.Compared with previously report,HPLC has lower detection limit and wider detection range,and at least 5 alcohol extractive components can be separated well from impurities with quantitative analyses of puerarin and daidzin.The rapidly and accurately qualitative and quantitative detecting of puerarin and daidzin in radix pueraria can be realized by TLC-HPLC under the optimal conditions.

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