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Position: Home > Articles > Rapid Determination of ATP,ADP and AMP Compounds in Pork by Ion-pair RP-HPLC Method Chinese Journal of Animal Science 2015,51 (21) 63-66

反相离子对色谱法快速测定猪肉中ATP和ADP及AMP的含量

作  者:
沈丽;郭慧;唐丽萍;贾唯暄
单  位:
北京物资学院物流学院
关键词:
高效液相色谱;ATP;ADP;AMP;猪肉
摘  要:
采用反相离子对色谱法建立了一种快速测定猪肉中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、单磷酸腺苷(AMP)含量的检测方法。采用Waters BEH C18柱作为色谱柱,以20 mmol/L磷酸钾缓冲溶液(含5 mmol/L四丁基溴化铵,pH=6.5)和乙腈作为流动相进行梯度洗脱,采用光二极管阵列检测器进行检测,检测波长为259 nm。结果表明:HPLC可以实现猪肉样品中ATP、ADP、AMP 3种物质的同步检测,平均回收率在80.89%~97.82%,相对标准偏差小于2.81%。
译  名:
Rapid Determination of ATP,ADP and AMP Compounds in Pork by Ion-pair RP-HPLC Method
作  者:
SHEN Li;GUO Hui;TANG Li-ping;JIA Wei-xuan;Logistics School,Beijing Wuzi University;
关键词:
HPLC;;ATP;;AMP;;ADP;;Pork
摘  要:
We achieved rapid determination of adenosine triphosphate(ATP),adenosine diphosphate(ADP) and adenosine monophosphate(AMP) in pork by using ion-pair reverse phase HPLC method.A Waters BEH C18 column was used as stationary phase.The solution containing 20 mmol/L potassium phosphate buffer and 5 mmol/L tetrabutyl ammonium bromide(pH 6.5) and acetonitrile was applied as mobile phase for gradient elution.Photodiode array detector was employed as a detector,and detection wavelength was fixed at 259 nm.The results showed that ATP.ADP and AMP could be simultaneously detected from pork samples,and the obtained average recoveries ranged from80.89%to 97.82%.The relative standard deviation was less than 2.81%.

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