作 者：
宋瑞瑞;包斌;卜永士;王永先;陈丽娟;吴文惠
单 位：
上海海洋大学食品学院;上海海洋大学海洋科学研究院;上海水产品加工与贮藏工程技术研究中心
关键词：
Ⅱ型胶原蛋白;限制性酶解;SDS-PAGE;氨基酸分析;紫外光谱分析
摘 要：
目的:对采用碱处理-胃酶水解方法分离的蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性进行研究.方法:蓝鲨软骨经冷冻干燥粉碎,顺次用碱处理、胃蛋白酶限制性水解、盐析和透析获得Ⅱ型胶原蛋白.采用SDS-PAGE、氨基酸成分分析、紫外光谱扫描和差示扫描量热法对提取的Ⅱ型胶原蛋白进行鉴定.结果:蓝鲨Ⅱ型胶原蛋白由分子质量为130kD的α1链构成,甘氨酸是蓝鲨Ⅱ型胶原蛋白含量最高的氨基酸为356‰,富含丙氨酸、脯氨酸和羟脯氨酸,且肽链氨基酸残基具有Gly-X-Y的构成特点,蓝鲨Ⅱ型胶原蛋白在226nm波长处有紫外吸收峰,变性温度为41℃.结论:采用碱处理-限制性酶水解分离得到蓝鲨Ⅱ型胶原蛋白,具有Ⅱ型胶原蛋白的典型特征.
单 位：
College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China%Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center,Shanghai 201306,China%Institute of Marine Science,Shanghai Ocean University,Shanghai 201306,China
关键词：
typeⅡ collagen%restriction proteolysis%SDS-PAGE%amino acid analysis%UV spectroscopic analysis
摘 要：
Objective: To isolate type Ⅱ collagen from Prionace glauca cartilage using alkali treatment/pepsin hydrolysis method,and to determine its biological activity.Methods: The freeze-dried Prionace glauca cartilage was crushed into 60200 mesh powder.Then,NaOH treatment and hydrolysis with pepsin were conducted.After salting and dialysis,the type Ⅱ collagen was obtained.SDS-PAGE,amino acid composition analysis,UV spectroscopic scanning and differential scanning calorimetry were applied to identify the type Ⅱ collagen.Results: Prionace glauca type Ⅱ collagen was composed of α1 chain and had the molecular weight of 130 kD.Glycine revealed the highest population in Prionace glauca type Ⅱ collagen with the ratio of 356‰.Meanwhile,type Ⅱ collagen from Prionace glauca was rich in alanine,proline and hydroxyproline.Moreover,type Ⅱ collagen from Prionace glauca had the characteristics of Gly-X-Y chain,UV absorption peak at 226 nm,thermal denaturation temperature of 41 ℃.Conclusion: Type Ⅱ collagen from Prionace glauca separated by alkali treatment/enzyme hydrolysis has typical characteristics of type Ⅱ collagen.
