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当前位置: 首页 > 文章 > 鸡肉鸡肝中20种磺胺类兽药残留的测定——QuEChERS-超高效液相色谱串联质谱法 当代畜牧 2016 (33) 44-46
Position: Home > Articles > Determination of 20 Sulfonamides Residues in Chicken and Chicken Liver-QuEChERS-UPLC-MS/MS Contemporary Animal Husbandry 2016 (33) 44-46

鸡肉鸡肝中20种磺胺类兽药残留的测定——QuEChERS-超高效液相色谱串联质谱法

作  者：
赵颖;李晓东;姜玲玲;刘瑜;金雁;徐宜宏;吴渺渺
单  位：
沈阳出入境检验检疫局
关键词：
鸡肉;鸡肝;磺胺药残留;液相色谱串联质谱
摘  要：
笔者建立了一种同时测定鸡肉及鸡肝中20种磺胺类兽药残留的Qu ECh ERS-超高效液相色谱串联质谱检测方法。样品经1%的乙酸乙腈水溶液(20/80,V/V)提取,振荡离心后,取上清液于Qu ECh ERS净化管中净化,取净化液,浓缩至干,甲醇水溶液(甲醇∶水=1∶4)溶解残渣,过膜,待测。采用C18色谱柱(2.1 mm×100 mm,1.7μm)分离,以0.2%甲酸水溶液和0.2%甲酸乙腈溶液作为流动相进行梯度洗脱,UPLC-MS/MS法同时定性、定量测定鸡肉和鸡肝中20种磺胺类兽药残留。20种磺胺类兽药在0.5 ng/m L~80 ng/m L范围内线性关系良好,相关系数(r)均大于0.99μg/kg。以5μg/kg、10μg/kg和50μg/kg 3个浓度水平进行添加回收实验,20种磺胺类兽药的平均回收率在64.4%~108.0%之间,相对标准偏差为2.6%~19.0%,方法的定量限为5μg/kg。方法重现性、灵敏度高,分析时间短,确证能力强,适用于鸡肉及鸡肝中20种磺胺类兽药残留的同时检测。
译  名：
Determination of 20 Sulfonamides Residues in Chicken and Chicken Liver-QuEChERS-UPLC-MS/MS
作  者：
Zhao Ying;Li Xiaodong;Jiang Lingling;Liu Yu;Jin Yan;Xu Yihong;Wu Miaomiao;
关键词：
chicken;;chicken liver;;sulfonamide;;UPLC-MS/MS
摘  要：
A method for simultaneous determination of 20 sulfonamides residues in chicken and chicken liver by Qu ECh ERS-UPLCMS/MS. Samples were extracted with 1% acetic acid in acetonitrile, and the extraction was centrifuged and purified in Qu ECh ERS kit,separated on a C18column(2.1 mm×100 mm, 1.7 μm). The determination of 20 sulfonamides residues in chicken and chicken livers by UPLC-MS/MS was carried out by gradient elution with 0.2% formic acid in water and 0.2% formic acid in acetonitrile as mobile phase.The correlation coefficients(r) of the 20 sulfonamides residues in the range of 0.5 ng/m L~80 ng/m L were all better than 0.99. The addition and recovery experiments were carried out at the concentration of 5 μg/kg,10 μg/kg,50 μg/kg, The average recoveries of 20 sulfonamides were 64.4% ~108.0%, the relative standard deviations were 2.6% ~19.0%. The limit of quantitation was 5 μg/kg. The reproducibility, high sensitivity, short analysis time and strong confirmation ability were suitable for the simultaneous determination of 20 sulfonamides residues in chicken and chicken livers.

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