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Position: Home > Articles > Screening and Identification of Cellulose-producing Strains FOOD SCIENCE 2010,31 (5) 211-214

细菌纤维素高产菌株的筛选及鉴定

作  者:
曹海鹏;袁帅;赵昆;张霞;胡承
单  位:
四川大学生命科学学院,生物资源与生态环境教育部重点实验室;四川大学生命科学学院
关键词:
细菌纤维素;葡糖醋杆菌;生理生化特征;鉴定
摘  要:
以残次的水果为原料,经静置富集培养、多次筛选纯化等步骤,分离出一株细菌纤维素产量较高且遗传性能稳定的菌株G-29。根据第二版《伯杰系统细菌学手册》和第九版《伯杰氏细菌鉴定手册》,对该菌株进行形态、生理生化特征和16SrDNA分子序列分析。初步鉴定该菌为葡糖醋杆菌属(Gluconacetobactersp.)一个新菌种或者中间葡糖醋杆菌(Gluconacetobacter intermedius)的一个亚种。菌株G-29已保藏于中国典型培养物保藏中心,保藏编号为CCTCCNO:M208234。
译  名:
Screening and Identification of Cellulose-producing Strains
作  者:
CAO Hai-peng,YUAN Shuai,ZHAO Kun,ZHANG Xia,HU Cheng (Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China)
关键词:
bacterial cellulose;Gluconacetobacter;physiological and biochemical characteristics;identification
摘  要:
Wounded fruits were used as raw materials to isolate and obtain a cellulose-producing strain, G-29, with high productivity and genetic stability through static enriching culture and repeated screening. The morphology, physiological and biochemical characteristics and 16S rDNA sequence of G-29 were analyzed. This strain was identified to be a new subspecies of Gluconacetobacter intermedius in the species of Gluconacetobacter. This strain has been preserved in China Center for Typical Culture Collection (CCTCC) with the collection number of CCTCC NO: M208234.

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