当前位置: 首页 > 文章 > 花染色体组型分析及细胞核DNA含量的测定 广东海洋大学学报 2008,28 (1) 11-14
Position: Home > Articles > Chromosome Karyotype and Nuclear DNA Contents of the Spotted Steed, Hemibarbus maculatus Journal of Guangdong Ocean University 2008,28 (1) 11-14

花染色体组型分析及细胞核DNA含量的测定

作  者:
顾若波;徐钢春;闻海波;许洪洪
单  位:
中国水产科学研究院淡水渔业研究中心
关键词:
花;染色体;核型;DNA含量
摘  要:
采用PHA、秋水仙碱腹腔或背部肌肉注射,活体培养法,取花肾细胞制成悬浮液,用空气干燥法制片。经Giemsa染色,镜检细胞分裂相,统计结果为2n=50,核型公式为:2n=20m+12sm+10st+8t,NF=82。同时,取花肌肉和肝脏为材料,以鸡血细胞为DNA标准(2.30pg/2c),使用EPICS-XL型流式细胞仪测定了花细胞DNA含量,分别为2.219±0.055pg/2c和2.214±0.083pg/2c。
译  名:
Chromosome Karyotype and Nuclear DNA Contents of the Spotted Steed, Hemibarbus maculatus
作  者:
GU Ruo-bo1,XU Gang-chun1,WEN Hai-bo1,Xu Hong-hong2 (1.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China; 2. Sihong Productivity Promotion Center of Jiangsu, Sihong 223900, China)
关键词:
Hemibarbus maculatus Bleeker; chromosomes; karyotype; DNA content
摘  要:
The metaphase chromosome of spotted steed (Hemibarbus maculatus) was prepared from kidney by PHA and colchicine injection and air dried. The karyotype was analyzed. The results showed that there were 50 telocentric chromosomes in spotted steed and its karyotype was 2n=20m+12sm+10st+8t, NF=82.Using DNA of chicken erythrocyte(2.30pg/2c)as a standard, diploid nucleus DNA contents of H. maculates were measured from the muscle and liver by flow cytometer (EPICS-XL). DNA content was 2.219±0.055pg/2c and 2.214±0.083 pg/2c respectively.

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