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Position: Home > Articles > Studies on the Technique of Tissue Culture and Rapid Propagation of Lily Journal of Henan Agricultural Sciences 2004,33 (5) 56-59

百合组培快繁技术研究

作  者:
王永江;张振臣;张丽芳;乔奇;靳秀兰
单  位:
河南省农业科学院植物保护研究所
关键词:
百合;组织培养;快繁
摘  要:
以食用百合鳞片作外植体 ,以MS为基本培养基 ,对其组培快繁技术进行了研究。结果表明 ,适宜的芽诱导培养基为MS + 1 .0mg/L 6-BA + 0 .1mg/LNAA + 1 .0mg/LKT ;适宜的快繁培养基为MS + 1 .0mg/L 6-BA + 0 .0 1mg/LNAA + 0 .5mg/LKT ;适宜的生根培养基为MS + 0 .1mg/LIBA ;芽诱导和快繁较适宜的条件为光照 1 6h/d、温度 ( 2 5± 1 )℃ ;在光照 1 2h/d、温度 ( 2 5± 1 )℃条件下较易生根
译  名:
Studies on the Technique of Tissue Culture and Rapid Propagation of Lily
作  者:
WANG Yong jiang,ZHANG Zhen chen *,ZHANG Li fang,QIAO Qi,JIN Xiu lan (Plant Protection Institute of Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
关键词:
Lily;Tissue culture;Rapid propagation
摘  要:
Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃.

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