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RP-HPLC法测定迷迭香药材中鼠尾草酸

作  者:
李海;李鹏辉;黄惠华;李佳银
单  位:
华南理工大学;湖南三福生物科技有限公司;湖南农业大学
关键词:
高效液相色谱;迷迭香;鼠尾草酸
摘  要:
为建立高效液相色谱法测定迷迭香中鼠尾草酸含量的方法,以乙腈-0.1%磷酸水(60∶40)为流动相,检测波长为230 nm;柱温30℃,等度洗脱,流速为1.0 m L/min。结果显示,鼠尾草酸在0.277 2~1.016 4 mg/m L范围具有良好的线性关系(R2=0.999 92),回收率为97.56%。该方法快速、灵敏、准确,目标峰分离度良好,可为迷迭香药材以及提取物的质量控制提供依据。
译  名:
Determination of Carnosic Acid in Rosemary by RP-HPLC
作  者:
LI Hai;LI Peng-hui;HUANG Hui-hua;LI Jia-yin;South China University of Technology;Hunan Agriculture University;Hunan Sunfull Bio-tech Co.,Ltd;
关键词:
HPLC;;Rosemarinus officinalis L.;;carnosic acid
摘  要:
To establish a HPLC method for the determination of carnosic acid in Rosemarinus officimalis L., the anaiysis was carried out on Pheromenex Ecosil C18 column(200 mm ×4.6 mm,5 μm), the mobile phase was composed of actonitrile and 0.1% phosphoric acid aqucous(60 ∶ 40, v/v).The detection wavelength was 210 nm, with flow rate 1.0 m L/min at column temperature of 30 ℃.The standard curve of linear relationship was of 0.277 2-1.016 4 mg/m L and R2=0.999 92, average recovery was 97.56%. The method was accurate, sensitive, simple and reliable for the determination of carnosic acid in Rosemarinus oficinalis L., suitable for the quality evaluation of Rosemarinus officinalis L.

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