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Position: Home > Articles > The analysis on BMP4 gene polymorphism and litter size in different population of sheep Journal of Agricultural University of Hebei 2012,35 (1) 70-74

不同绵羊群体BMP4基因多态性与产羔数关系分析

作  者:
任立坤;刘月琴;张英杰;王红娜;杨少华;董李学
单  位:
河北农业大学动物科学技术学院;唐山市畜牧水产品质量监测中心
关键词:
BMP4基因;小尾寒羊;无角陶赛特;产羔数
摘  要:
以BMP4基因为候选基因,采用PCR-SSCP方法,分析了小尾寒羊和无角陶赛特及其杂交F1代、F2代的BMP4基因的不同基因型频率,并对该位点不同基因型与产羔数之间的关系进行了分析。结果显示:小尾寒羊BB、B+、++基因型频率分别是1.0%、29.7%、69.2%;无角陶赛特BB、B+、++型频率分别为0、80.8%、19.2%;陶寒F1代BB、B+、++基因型频率分别为5.3%、39.4%、55.3%;陶寒F2代BB、B+、++型基因型频率分别为0、5.0%、95.0%。小尾寒羊的基因型++、B+、BB平均产羔数分别为(1.00±0.00)、(1.67±0.11)、(2.40±0.13),经χ2检验,差异极显著(P<0.01);陶寒F1代的基因型++、B+、BB平均产羔数分别为(2.00±0.00)、(2.45±0.53)、(2.00±0.00),经χ2检验,++、BB组与B+组的平均产羔数间的差异极显著(P<0.01);B基因不能提高无角陶塞特母羊产羔数。
译  名:
The analysis on BMP4 gene polymorphism and litter size in different population of sheep
作  者:
REN Li-kun1,LIU Yue-qin1,ZHANG Ying-jie1,WANG Hong-na1,YANG Shao-hua1,DONG Li-xue2 (1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China; 2.The Livestock and Aquatic Products Quality Inspection and Test Center,Tangshan 063000,China)
关键词:
BMP4 gene;small tailed han sheep;poll dorset;litter size
摘  要:
Bone morphogenetic protein 4(BMP4)gene was detected in both Small Tailed Han sheep,Dorset sheep,F1 generation sheep(Small Tailed Han sheep×Dorset sheep) and F2 generation sheep(F1 generation sheep×Dorset sheep)by PCR-SSCP as a candidate gene for the prolificacy of sheep,analyzed the relationship between the sites of different genotypes and litter size.The results indicated that the genotype frequencies of BB,B+ and ++ of Small Tailed Han sheep were 1.0%,29.7% and 69.2% respectively;the genotype frequencies of B+ and ++ of Dorset sheep were 80.8% and 19.2% respectively;the genotype frequencies of BB,B+ and ++ of F1 generation sheep were 5.3%,39.4% and 55.3% respectively;the genotype frequencies of BB,B+ and ++ of F2 generation sheep were 0,5.0% and 95.0%.The average litter size of the genotype of ++,B+ and BB were(1.00±0.00),(1.67±0.11) and(2.40±0.13),and the difference was extremely significant tested by χ2 analysis(P<0.01).the genotype of ++,B+ and BB of F1 generation were(2.00±0.00),(2.45±0.53) and(2.00±0.00),and the difference of ++,BB and B+ was extremely significant(P<0.01);gene B can't increase the litter size of Dorset sheep.

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