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当前位置: 首页 > 文章 > 石油降解菌筛选鉴定及耐受性分析 东北农业大学学报 2017,48 (1) 49-57
Position: Home > Articles > Screening and identification of oil degrading bacteria and tolerance analysis Journal of Northeast Agricultural University 2017,48 (1) 49-57

石油降解菌筛选鉴定及耐受性分析

作  者：
刘宇程;王姗镒;马丽丽;陈明燕;张红杰;杨国通
单  位：
中海油能源发展股份有限公司工程技术分公司油田作业支持公司;中联煤层气有限责任公司;西南石油大学化学化工学院
关键词：
石油降解菌;筛选;鉴定;耐受性分析
摘  要：
试验从污泥中筛选3株高效石油烃降解菌m_1,m_2,m_3,对原油最高降解效率达73%,16S rDNA鉴定结果表明,m_1为Citrobacter柠檬酸细菌属,m_2为Tatumella塔特姆菌属,m_3为Kluyvera克吕沃尔氏菌属。分析3株石油降解菌耐受性,结果表明,m_2耐强碱性;m_1、m_2耐较高浓度盐;m_2在高浓度Cr中生长;m_3在高浓度Hg、Cd、Pb中较好生长。
译  名：
Screening and identification of oil degrading bacteria and tolerance analysis
作  者：
LIUYucheng;WANG Shanyi;MA Lili;CHEN Mingyan;ZHANG Hongjie;YANG Guotong;School of Chemistry and Chemical Engineering,Southwest Petroleum University;China United Coalbed Methane Corporation,Ltd.;CNOOC Energy Development Limited by Share Ltd.Engineering and Technology Branch Oil Field Operations Support Company;
关键词：
petroleum degrading bacteria;;screening;;identification;;tolerance analysis
摘  要：
Three strains of efficient hydrocarbon degrading bacteria m_1, m_2, m_3, were screened from sludge, the highest degradation efficiency of crude oil can reach 73%, and through the 16 S rDNA identification, the identification results showed m_1 was similar to the Citrobacter, m_2 was similar to the Tatumella, m_3 was similar to the Kluyvera. Then tolerance analysis of the three strains was followed and the results showed that m_2 could resist strong alkaline, m_1, m_2 were resistant to high concentration of salt, m_2 could grow in high concentration of Cr, and m_3 grew well in high concentration of Hg, Cd, Pb.

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