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当前位置: 首页 > 文章 > 一株镍抗性和石油烃降解菌的分离鉴定及其生物学特性 生物技术通报 2017,33 (10) 169-177
Position: Home > Articles > Isolation and Identification of a Nickel-resistant and Petroleum Hydrocarbon Degrading Strain and Its Biological Characteristics Biotechnology Bulletin 2017,33 (10) 169-177

一株镍抗性和石油烃降解菌的分离鉴定及其生物学特性

作  者：
马永松;李琋;李珍珍;王佩洁
单  位：
西南石油大学化学化工学院
关键词：
石油降解菌;镍抗性;分离鉴定;生物学特性
摘  要：
从四川省长宁-威远地区页岩气开发井场重度污染区的土壤中,筛选出一株镍抗性和石油高效降解菌株,研究其生物学特性和部分生理生化指标。将种子液接种在原油重金属液体培养基中,30℃、130 r/min条件下分别培养7 d探究该菌株对金属镍离子的抗性和对总石油烃(Total petroleum hydrocarbon,TPH)的降解率,以及经生理生化实验、形态观察和16S r DNA对该菌株鉴定分析。结果显示,该菌株对Ni2+的耐受性可达300 mg/L,对Ni2+的去除率和吸附率分别达到了56.64%和52.16%,同时对总TPH的降解率达到35.65%。确定该菌种属柠檬酸杆菌属(Citrobacter sp.),命名为Citrobacter farmeri strain M1。该菌株的最适生长温度为30℃,最适生长p H范围为7-9,最适C∶N为10∶1和盐最高耐受度可达5%。该菌株有较好的石油降解能力并对金属镍有较强的抗性,适用于石油重金属混合污染土壤的修复。
译  名：
Isolation and Identification of a Nickel-resistant and Petroleum Hydrocarbon Degrading Strain and Its Biological Characteristics
作  者：
MA Yong-song;LI Xi;LI Zhen-zhen;WANG Pei-jie;School of Chemistry and Chemical Engineering,Southwest Petroleum University;
关键词：
petroleum-degrading bacterium;;nickel resistance;;isolation and identification;;biological characteristic
摘  要：
A bacterial strain with high ability of nickel resistance and petroleum-degrading was isolated from the soil of heavily-pollutedareas around the shale gas wells in Changning-Weiyuan,Sichuan Province,and its biological characteristics and physiological-biochemicalindexes were studied. The seed liquid was inoculated into the liquid medium containing heavy metal and crude oil,cultured at 30℃ and 130 r/min for 7 days to explore the resistance of the strain to nickel ions(Ni2+)and the rate of degrading total petroleum hydrocarbon(TPH).Further,the strain was identified based on the experimental results of physiology and biochemistry,morphological observation and 16 Sr DNA sequences. As results,the tolerance of the strain to Ni2+ reached 300 mg/L,and the removal rate and adsorption rate of Ni2+ reached56.64% and 52.16%,respectively; meanwhile,the degradation rate of TPH reached 35.65%. The species of the strain belonged to Citrobacter(Citrobacter sp.)and named Citrobacter farmeri strain M1. The optimal growth temperature of this strain was 30℃,the optimal growth p H wasfrom 7 to 9,the optimal C∶N was 10∶1,and the maximum salt tolerance was up to 5%. In conclusion,solid nickel resistance and petroleumdegradability of the strain contributes to its applicability in the remediation of heavy metal and oil-contaminated soil.

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