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Position: Home > Articles > EFFECT OF DENSE PHASE CARBON DIOXIDE ON MEMBRANE PERMEABILIZATION OF E.coli Journal of Nuclear Agricultural Sciences 2012,26 (1) 91-96

高密度CO_2处理对E.coli细胞膜渗透性的影响

作  者:
王莹莹;张德权;李淑荣;李娟;饶伟丽
单  位:
中国农业科学院农产品加工研究所/农业部农产品加工与质量控制重点开放实验室
关键词:
高密度CO2;E.coli;细胞膜渗透性
摘  要:
以E.coli菌悬液为研究对象,通过测定高密度CO2处理(DPCD)后E.coli上清液中蛋白质、核酸、Mg2+、K+离子和丙二醛的含量,辅助透射电镜观察,研究DPCD对E.coli细胞膜渗透性的影响。在7MPa、37℃条件下,E.coli经高密度CO2处理10min后,99%以上的E.coli失活,同时研究发现蛋白质、核酸及Mg2+、K+离子等胞内物质均发生了不同程度的泄漏,丙二醛含量增加,E.coli胞内物质密度降低。密度CO2处理造成E.coli细胞膜渗透性的增加,这也是导致E.coli死亡的原因之一。
译  名:
EFFECT OF DENSE PHASE CARBON DIOXIDE ON MEMBRANE PERMEABILIZATION OF E.coli
作  者:
WANG Ying-ying ZHANG De-quan LI Shu-rong LI Juan RAO Wei-li(Key Laboratory of Agricultural Product Processing and Quality Control,Ministry of Agriculture/Institute of Agro-Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193)
关键词:
dense phase carbon dioxide;E.coli;membrane permeabilization
摘  要:
To study the effects of dense phase carbon dioxide(DPCD) on membrane permeability of E.coli,the content of protein,nucleic acid,Mg2+,K+ and malondialdehyde(MDA) of E.coli were studied coupled with Transmission Electron Micrographs(TEM) technique.Under the DPCD condition of 7MPa,37℃ for 10min,99% of E.coli was inactivated.After DPCD treatment,proteins,nucleic acid and Mg2+,K+ leaked from cells,and the content of MDA increased and the density of substances inside the cell decreased through the TEM view.The results indicated that DPCD treatment caused the membrane permeability of E.coli increased which was also an important cause of cell death.

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