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Position: Home > Articles > Research on the Extract Separation Purification and Antioxidant Activity of Tea Polyphenols from Jasmine Tea Food and Nutrition in China 2010 (04) 47-51

茉莉花茶中茶多酚的提取分离纯化及其抗氧化性能研究

作  者:
张仁堂;谷端银;黄守耀
单  位:
山东农大肥业科技有限公司;山东农业大学食品科学与工程学院
关键词:
茉莉花茶;茶多酚;分离纯化;抗油脂氧化
摘  要:
本实验采用溶剂法浸提茉莉花茶中茶多酚,研究发现,相对最优的工艺条件为:温度70℃,乙醇溶液浓度60%,提取次数1次,提取时间60min。在此浸提工艺下提取到茶多酚粗提物后用石油醚、氯仿和乙酸乙酯萃取,得乙酸乙酯相过聚酰胺柱分离纯化,将得到的不同洗脱组分过高效液相色谱检测发现:30%乙醇洗脱组分含表儿茶素没食子酸酯(ECG)最高,60%乙醇洗脱组分中含有表没食子儿茶素没食子酸酯(EGCG),95%乙醇洗脱组分主要是儿茶素没食子酸酯(CG)。继而对不同组分进行抗氧化活性研究得出:60%洗脱组分对油脂的抗氧化活性最高;30%洗脱组分与60%洗脱组分对DPPH自由基50%清除率的浓度分别为0.1723mg/ml、0.1693mg/ml。
译  名:
Research on the Extract Separation Purification and Antioxidant Activity of Tea Polyphenols from Jasmine Tea
作  者:
ZHANG Ren-tang1,HUANG Shou-yao1,HAO Xiao-ran1,GU Duan-yin2 (1College of Food Science and Engineering, Shandong Agricultural University1, Taian 271018; 2Shan Dong Nong Da Fertilizer Science and Technology Limited Corporation, Taian 271000)
关键词:
Jasmine Tea;Tea Polyphenols;Separation and Purification;Antioxidant Fat
摘  要:
Tea polyphenols was the tea polyhydroxy mixture of phenolic compounds. This was a solvent extraction experiment with jasmine tea. The relatively optimal conditions were temperature 70℃,the concentration of ethanol solution of 60%,extract 1 time for 60min. The highest content of polyphenols was the use of ethanol extraction followed by petroleum ether, ethyl acetate and chloroform extraction, ethyl acetate phase over a polyamide column separation and purification with 30%, 60%, 95% of the ethanol eluate elution respectively, the eluate would be tested over liquid chromatography with HPLC conditions 30% ethanol eluate contains epicatechin gallate (ECG), 60% ethanol eluate contained epigallocatechin gallate (EGCG), 95% ethanol eluate eluting fluid catechin gallate (CG) and 60% fat-eluting system the highest antioxidant activity. 30% elution system and 60% elution system of 50% of DPPH free radical clearance rate of the concentration of 0.1723mg/ml, 0.1693mg/ml.

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