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Position: Home > Articles > Mitotic Karyotyping and Meiotic Observation in Hibiscus moscheutos L. Journal of Plant Genetic Resources 2009,10 (2) 286-289

芙蓉葵(Hibiscus moscheutos L.)有丝分裂核型分析及减数分裂观察

作  者:
刘坤;王省芬;迟吉娜;张桂寅;吴立强;李志坤;马峙英
单  位:
河北农业大学/河北省作物种质资源重点实验室
关键词:
芙蓉葵;染色体;有丝分裂;核型;减数分裂
摘  要:
芙蓉葵是棉属的近缘植物,具有棉花需要的早熟、强结实、耐寒、抗黄萎病等优良性状。本文对芙蓉葵的细胞学特征进行了研究,为其优良性状在棉花遗传改良中的应用提供细胞遗传学资料和理论依据。利用压片法获得了分散良好、形态清晰的有丝分裂中期染色体,核型分析结果表明:芙蓉葵染色体的平均长度为5.96±0.83μm,在1号染色体上发现随体;臂比>2的染色体占10.5%,最长染色体与最短染色体的比值为1.68,核型类型为2A型,核型公式为2n=2x=38=32m+6 sm(2SAT)。芙蓉葵花粉母细胞的减数分裂正常,中期Ⅰ有19个二价体(19Ⅱ)。
译  名:
Mitotic Karyotyping and Meiotic Observation in Hibiscus moscheutos L.
作  者:
LIU Kun,WANG Xing-fen,CHI Ji-na,ZHANG Gui-yin,WU Li-qiang,LI Zhi-kun,MA Zhi-ying (Key Laboratory of Crop Germplasm Resources of Hebei/Agricultural University of Hebei,Baoding 071001)
关键词:
Hibiscus moscheutos L.;Chromosome;Mitosis;Karyotype;Meiosis
摘  要:
Hibiscus moscheutos L.is the relative of Gossypium L.It has some eminent traits,such as earliness,good fecundity,cold-tolerance and Vertcillium wilt resistance and so on.In this paper,the well-spread and clear mitotic metaphase chromosomes of Hibiscus moscheutos were obtained.The karyotype analysis indicated that there was a pair of satellites on chromsome No.1,and the average length of Hibiscus moscheutos's chromosomes was 5.96±0.83μm.The arm ratios of 10.5% chromosomes were over 2,and the ratio of the longest chromosome to the shortest one was 1.68.The karyotype was 2A and the karyotype formulae was 2n=2x=38=32m+6sm(2SAT).No abnormal behavior was observed in the meiotic process of pollen mother cell of Hibiscus moscheutos.There were 19 bivalents(19Ⅱ)at metaphaseⅠ.

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