中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 吉林芦花鸡IGF-2基因多态位性与生产性能相关分析 东北农业科学 2023 (6) 101-106
Position: Home > Articles > The Measurement and Correlation Analysis of Polymorphism on IGF-2 gene in Barred Plymouth Rocks from Jilin Province Journal of Northeast Agricultural Sciences 2023 (6) 101-106

吉林芦花鸡IGF-2基因多态位性与生产性能相关分析

作  者：
张英惠;栾祜;陈小辛;柳俭强;刘艺;李旭;安鼎杰;张立春
单  位：
吉林省农业科学院动物生物技术研究所;吉林农业大学动物科学技术学院
关键词：
吉林芦花鸡;吉林矮小芦花鸡;IGF-2基因;多态性;生长性状
摘  要：
为探究吉林芦花鸡IGF-2基因遗传多样性及与生长性能相关性，本试验通过RT-PCR方法克隆正常吉林芦花鸡和矮小吉林芦花鸡肝脏组织IGF-2基因并进行SNP筛选，再通过特定单碱基多态位点（Single-nucleotide Polymorphisms,SNP）高分辨率溶解曲线（High Resolution Melting analysis, HRM）法对7个不同地方鸡群体进行分析，最后对正常芦花鸡和矮小芦花鸡生产性能进行相关分析。RT-PCR表明IGF-2在两类鸡肝脏组织表达，测序发现c.345 T>C和3'UTR19 G>A突变。不同群体c.345 T>C位点遗传多样性分析发现除寿光鸡群体TC基因型为优势基因型外，其他群体优势基因型均为TT型。其中汶上芦花鸡群体中完全不存在T>C突变，黄鸡和油鸡群体中亦未检测到CC基因型。Hardy-Weinberg平衡检验发现该位点在芦花鸡、矮小芦花鸡、黄鸡和寿光鸡群体处于不平衡状态。矮小芦花鸡群体生长性能相关分析发现母鸡中TT型个体体重显著高于TC型个体（P<0.05），公鸡TT和TC基因型个体胸深极显著高于CC基因型个体（P<0.01）。吉林芦花鸡IGF-2基因多态位点及其与生产性能相关性的发现为利用该位点进行群体内选育奠定理论基础。
译  名：
The Measurement and Correlation Analysis of Polymorphism on IGF-2 gene in Barred Plymouth Rocks from Jilin Province
作  者：
ZHANG Yinghui;LUAN Hu;CHEN Xiaoxin;LIU Jianqiang;LIU Yi;LI Xu;AN Dingjie;ZHANG Lichun;Institute of Animal Biotechnology,Jilin Academy of Agricultural Sciences;Institute of Animal Science and Technology,Jilin Agricultural University;
关键词：
Jilin barred plymouth rocks;;Jilin dwarf barred plymouth rocks;;IGF-2;;Polymorphism;;growth traits
摘  要：
To investigate the polymorphism and correlation of the IGF-2 gene to growth traits in Jilin Barred Plymouth Rocks, this study utilized RT-PCR to clone IGF-2 mRNA from liver tissues of normal and dwarf Barred Plymouth Rocks. The amplification products were sequenced, and SNP sites were identified. One SNP was chosen, and the polymorphism was assessed in seven local chicken using High-Resolution Melting Curve(HRM) analysis. Furthermore, the correlation to growth traits in normal and dwarf Barred Plymouth Rocks was tested by One-Way ANOVA. The results of RT-PCR indicated that IGF-2 mRNA were normally expressed in the livers from both normal and dwarf individuals. Sequencing data revealed the existence of two SNP sites c.345 T>C and 3'UTR19 G>A.The polymorphism analysis of c.345 T>C in seven local populations demonstrated that the TT genotype was dominant in almost population, except for Shouguang chicken, where TC genotype was dominant. It was also observed that Wenshang Barred Plymouth Rocks population did not display any mutation at the site, while Beijing oil and Yellow chicken population did not have CC genotype. The Hardy-Weinberg equilibrium test indicated that the normal and dwarf chicken populations, as well as Yellow chicken and Shouguang chicken populations, were in disequilibrium. Correlation analysis between genotype and growth traits in dwarf chicken populations revealed that, in the dwarf chicken hen population, individuals with TT genotype exhibited significantly higher body weight compared to TC individual(P<0.05). In cock population, individuals with both the TT and TC genotype showed significantly greater on chest depth than those with CC individual(P<0.01). In conclusion, the discovery of polymorphism and their correlation with growth traits in IGF-2 gene provide theoretical evidences for improve the growth traits of local Barred Plymouth Rocks.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46