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当前位置: 首页 > 文章 > 瑞士乳杆菌蛋白酶水解乳清蛋白制备ACE抑制肽的条件优化 食品科学 2009,30 (11) 138-143
Position: Home > Articles > Optimization of Hydrolysis Conditions for Production of Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptide from Whey Protein Using Proteinases of Lactobacillus helveticus FOOD SCIENCE 2009,30 (11) 138-143

瑞士乳杆菌蛋白酶水解乳清蛋白制备ACE抑制肽的条件优化

作  者：
潘道东;郭宇星
单  位：
南京师范大学乳品生物技术研究所;宁波大学生命科学与生物工程学院
关键词：
ACE抑制肽;乳清蛋白水解;响应面法
摘  要：
目的:本实验以WPC-80乳清浓缩蛋白为原料,对瑞士乳杆菌蛋白酶水解乳清蛋白产生血管紧张素转移酶(ACE)抑制肽工艺条件进行研究。方法:通过单因素条件和响应面方法研究水解pH值、水解温度、酶与底物比、底物浓度和水解时间对水解度和ACE抑制率的影响。结果:研究发现,五个工艺条件对ACE抑制肽的产生都有影响,通过响应面法分析,确定瑞士乳杆菌蛋白酶酶解乳清蛋白的最佳水解条件为:[E]/[S]0.6%、底物浓度6%、pH9.18、温度38.90℃,时间8.0h,在此条件下水解,产物ACE抑制率达到92.21%,IC50为0.375mg/ml,水解度为18.76%。结论:应用响应面方法优化水解工艺条件是可行的。
译  名：
Optimization of Hydrolysis Conditions for Production of Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptide from Whey Protein Using Proteinases of Lactobacillus helveticus
作  者：
PAN Dao-dong1,2,GUO Yu-xing1 (1. Institute of Dairy Biotechnology, Nanjing Normal University, Nanjing 210097, China; 2. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China)
关键词：
ACE inhibitory peptide;whey protein hydrolysis;response surface methodology
摘  要：
Objective: To optimize the preparation technology of ACE inhibitory peptide from WPC-80 whey protein concentrate hydrolyzed by the proteases produced by Lactobacillus helveticus using response surface methodology. Methods: Through single- factor test and response surface methodology, the effects of five enzymatic hydrolysis conditions including temperature, pH, enzyme to substrate ([E]/[S]) ratio, substrate concentration and time on the degree of hydrolysis (DH) of WPC-80 whey protein concentrate and the ACE inhibitory activity were investigated. Results: All of five factors affected the production of ACE inhibitory peptide, and among them temperature, pH and [E]/[S] ratio were the key factors. The optimum temperature, pH, [E]/[S] ratio, substrate concentration and time were 38.90 ℃, 9.18, 0.6%, 6% and 8.0 h, respectively. Under these conditions, the ACE inhibitory activity was 92.21%, its IC50 was 0.375mg/ml, and the DH was 18.76%. Conclusion: The response surface methodology is feasible to optimize the enzymatic hydrolysis conditions for ACE inhibitory peptide preparation.

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