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当前位置: 首页 > 文章 > 晒黄烟种质资源遗传多样性分析与评价 植物遗传资源学报 2016,17 (2) 252-256
Position: Home > Articles > Analysis on Genetic Diversity of Yellow Sun-cured Tobacco Germplasm Resources Journal of Plant Genetic Resources 2016,17 (2) 252-256

晒黄烟种质资源遗传多样性分析与评价

作  者：
刘艳华;向德虎;闫宁;黄谨;首安发;杜咏梅;董建新
单  位：
广西区烟草公司贺州市公司;中国农业科学院烟草研究所
关键词：
晒黄烟;SSR;遗传多样性
摘  要：
为加强晒黄烟资源的收集保存与利用,本研究对来源于国家烟草中期库的6份晒黄烟和新收集的26份晒黄烟进行了SSR分子标记研究,根据来源不同将32份种质分成Z(资源库)、G(广西贺州)以及M(广西百色)3个群体。结果表明,(1)106对SSR引物共检测到110个位点,其中85个为多态性位点,多态性比率为77.27%,种质间的遗传相似系数变异范围为0.49~0.95,表明种质间遗传基础比较狭窄。(2)3个群体内遗传差异由大到小分别为:Z>M>G。Z与M的遗传相似系数为0.7762,遗传距离相对较远,G与M遗传相似系数为0.8963,遗传距离较近。(3)在相似系数0.49处可将32份种质分成2类,第1类包括寸三皮和大宁旱烟籽,第2类包括3个亚类,其中来源于Z群体的公会晒烟与新收集晒黄烟品种(系)归为一类,Z群体的其他种质与新收集种质遗传距离较远。(4)来自不同地点的同名种质大宁烟遗传距离相对较远,相似系数为0.788。不同名称的公会包盖烟和凤凰黄烟遗传相似性最高,相似系数为0.939。
译  名：
Analysis on Genetic Diversity of Yellow Sun-cured Tobacco Germplasm Resources
作  者：
LIU Yan-hua;XIANG De-hu;YAN Ning;HUANG Jin;SHOU An-fa;DU Yong-mei;DONG Jian-xin;Tobacco Research Institute of Chinese Academy of Agricultural Sciences;Hezhou Filiale of Guangxi Tobacco Corporation;
关键词：
yellow sun-cured tobacco;;SSR;;genetic diversity
摘  要：
In order to promote collection,conservation and utilization of yellow sun-cured tobacco resource,32 yellow sun-cured tobacco germplasm materials were analyzed using 106 pairs of microsatellite( SSR) markers. Based on origins,the tobacco germplasm resources were divided into three groups,including Z from Tobacco Mid-term Germplasm Repository,G from Hezhou of Guangxi Province and M from Baise of Guangxi Province. The results were as follows:( 1) Totally 110 loci were detected using the 106 pairs of SSR selected from 740 primer combinations,85 of these were polymorphic loci( 77. 27%). The range of variant coefficient was 0. 49-0. 95 based on molecular clustering,suggesting the genetic basis of the yellow sun-cured tobacco germplasm resources was narrow.( 2) The order of genetic diversity of the three groups was Z > M > G. The genetic coefficient was 0. 7762 between Z and M,and0. 8963 between G and M.( 3) The 32 tobacco germpasm materials were clastered into 2 groups at the position of coefficient of 0. 49. The first group included Cunsanpi and Daninghanyanzi germplasm resources. The second group included 3 sub-groups. Gonghuishaiyan from group Z was clustered together with the new collections while other tobacco germplasm resources weren't clustered with the new collections.( 4) The genetic distance of the homonymic germplasm resource Daningyan from different origins was far,with a coefficient of 0. 788. The genetic distance between Gonghuibaogaiyan and Fenghuanghuangyan was the closest with the coefficient of 0. 939.

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