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当前位置: 首页 > 文章 > 6-BA对中国石竹雄性不育植株离体保存的影响 现代农业科技 2011 (2) 223-224+226
Position: Home > Articles > Effect of 6-BA on Male Sterlity Plant in Vitro Propagation of Dianthus chinensis Modern Agricultural Science and Technology 2011 (2) 223-224+226

6-BA对中国石竹雄性不育植株离体保存的影响

作  者：
费永俊;汤隆;刘雅丽
单  位：
长江大学园艺园林学院
关键词：
中国石竹;6-BA;离体繁殖;雄性不育
摘  要：
该研究以中国石竹2个品种的雄性不育植株带芽茎段为初始外植体进行组织培养,筛选了适合其芽诱导、继代增殖的培养基配方,并对试管苗移栽介质进行了挑选,成功地建立了2个中国石竹雄性不育植株的离体繁殖与保存体系。结果表明:中国石竹芽的最佳诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,最佳继代增殖培养基为MS+6-BA 0.3 mg/L+NAA 0.1 mg/L,最适宜的试管苗移栽基质是珍珠岩。同时,2个不育材料组织培养繁殖后代仍表现为雄性不育,确保了原材料的雄性不育性状的稳定性。
译  名：
Effect of 6-BA on Male Sterlity Plant in Vitro Propagation of Dianthus chinensis
作  者：
FEI Yong-jun TANG Long LIU Ya-li(College of Horticulture and Garden,Yangtze University,Jingzhou Hubei 434025)
关键词：
Dianthus chinensis;6-BA;in vitro propagation;male sterility
摘  要：
Stem nodal segments of male sterile plants of two cultivars were used as the first explants for tissue culture and micropropagation of Dianthus chinensis.The objects of the study were to select the optimum medium for adventitious bud induction,proliferation and transplanting,and eventually establish the technique system of tissue culture.The results showed that the optimum medium for adventitious bud induction and proliferation were MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,MS+6-BA 0.3 mg/L+NAA 0.1 mg/L,respectively.The optimum transplanting substrate was perlite.Then the flower architecture of transplanted plants was observed,and confirmed that the characteristic of male sterility was retained.

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