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当前位置: 首页 > 文章 > 同时测定6种柿叶黄酮类物质方法的建立 山东农业科学 2016,48 (5) 131-136
Position: Home > Articles > Establishment of Simultaneous Detection Method of Six Flavonoids from Persimmon Leaves Shandong Agricultural Sciences 2016,48 (5) 131-136

同时测定6种柿叶黄酮类物质方法的建立

作  者：
安淼;余贤美;王洁;刘加芬;艾呈祥
单  位：
山东省果树研究所
关键词：
柿叶;黄酮类物质;高效液相色谱;同时检测
摘  要：
为建立一种同时测定6种柿叶黄酮类物质的方法,通过对液相色谱检测波长、流动相、柱温等条件的探索,最终确定了芦丁、山柰素、异鼠李素、槲皮素、异槲皮苷和紫云英苷6种黄酮类物质标准品的液相色谱检测条件:以十八烷基硅烷键合硅胶为填充剂,以甲醇为流动相A,以0.5%乙酸溶液为流动相B,进行梯度洗脱,0~100 min A∶B为20∶80,105 min后为40∶60;检测波长为360 nm;柱温为35℃;体积流量1.0 m L/min。并以两个品种的柿叶为样品,对所建立的方法进行验证,得到了预期结果,说明建立的检测方法是可行的。
译  名：
Establishment of Simultaneous Detection Method of Six Flavonoids from Persimmon Leaves
作  者：
An Miao;Yu Xianmei;Wang Jie;Liu Jiafen;Ai Chengxiang;Shandong Institute of Pomology;
关键词：
Persimmon leaves;;Flavonoid;;HPLC;;Simultaneous detection
摘  要：
The HPLC method for simultaneous detection of 6 flavonoids from persimmon leaves was established by exploring the detection conditions such as wavelength,mobile phase and column temperature.The 6 flavonoids were lutin,kaempferol,isorhamnetin,quercetin,isoquercitrin and astragalin. The results showed that the 6 flavonoid standards could be detected simultaneously under the following conditions: using C18 as stuffing bulking agent,carbinol as mobile phase A and 0. 5% acetic acid as mobile phase B; the samples were gradiently eluted at the ratios of phase A∶ B as 20∶ 80 in 0 ~ 100 minutes and 40∶ 60 after 105 minutes; and the detection was carried out with 360 nm of wavelength,35℃ of column temperature and 1. 0m L / min of volume flow rate. The detection method was verified with the leaves of two persimmon varieties,and the expected results were obtained,which indicated that the established method was feasible.

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