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当前位置: 首页 > 文章 > 可可FAT基因家族进化及表达模式分析 热带作物学报 2020 (5) 947-954
Position: Home > Articles > Phylogeny and Expression Profile of Fatty Acyl-ACP Thioesterase(FAT) Gene Family in Cacao(Theobroma cacao L.) Chinese Journal of Tropical Crops 2020 (5) 947-954

可可FAT基因家族进化及表达模式分析

作  者：
李付鹏;邓云梅;伍宝朵;秦晓威;闫林;赖剑雄
单  位：
关键词：
可可;酰基载体蛋白硫酯酶(FAT);系统进化;表达模式
摘  要：
在植物中,酰基-ACP硫酯酶(fatty acyl-ACP thioesterase, FAT)是调控脂肪酸合成的关键酶。为解析可可FAT基因家族成员的特点与功能,本研究从可可基因组中筛选鉴定出FAT基因家族的6个成员,分别命名为TcFATA、TcFATB1、TcFATB2、TcFATB3、TcFATB4、TcFATB5。6个基因外显子数目为6～7个,编码区(CDS)长度介于1128～1263 bp,预测蛋白分子量介于42.72～46.47 kDa,等电点介于6.57～9.10。进化分析结果表明可可FAT基因家族分成FATA与FATB亚群,FATA亚群包含1个可可TcFATA成员,FATB亚群包含5个可可TcFATBs成员。不同可可种子发育时期表达分析结果表明:TcFATA和TcFATB1伴随果实发育成熟,表达量呈下降趋势,TcFATA和TcFATB1在不同种质中表达量与油酸(C18:1)和棕榈酸(C16:0)比例呈正相关,表明其与脂肪酸组分比例调控有紧密关联。
译  名：
Phylogeny and Expression Profile of Fatty Acyl-ACP Thioesterase(FAT) Gene Family in Cacao(Theobroma cacao L.)
作  者：
LI Fupeng;DENG Yunmei;WU Baoduo;QIN Xiaowei;YAN Lin;LAI Jianxiong;Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops;College of Tropical Crop Science, Yunnan Agricultural University;
关键词：
Theobroma cacao;;fatty acyl-ACP thioesterase(FAT);;phylogenetic;;expression profile
摘  要：
Fatty acyl-ACP thioesterase(FAT) is the key enzyme regulating the synthesis of plant lipid. In order to better understand the characteristics of FAT gene family in Theobroma cacao, six novel FAT genes were identified from economically important cacao trees, designated as TcFATA, TcFATB1, TcFATB2, TcFATB3, TcFATB4, TcFATB5, respectively. Sequences analyses revealed that CDS of TcFATs was 1128–1263 bp, containing 6–7 exons. The molecular weight of the six predicted protein was 42.72–46.47 kDa, and pI of the proteins was 6.57–9.10. FAT family could be divided into FATA(one TcFATA) and FATB(five TcFATBs) subfamily. The expression patterns of the genes were investigated via real-time PCR in various developmental phases. The transcription level of TcFATA and TcFATB1 was continuously decreased along with pod development. Furthermore, the expression level of TcFATA and TcFATB1 was positively associated with the ratio of oleic acid(C18:1) and palmitic acid(C16:0) respectively. The results indicate that TcFATA and TcFATB1 are significantly related with the regulation of fatty acid component.

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