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当前位置: 首页 > 文章 > 一株耐盐菌的筛选、鉴定及对苯酚和镉耐性特征 安徽农业大学学报 2020 (3) 402-408
Position: Home > Articles > Screening, identification and tolerance to phenol and cadmium of a salt-tolerant strain Journal of Anhui Agricultural University 2020 (3) 402-408

一株耐盐菌的筛选、鉴定及对苯酚和镉耐性特征

作  者：
刘亚楼;樊霆;刘如;潘丹丹;郭艺慧;罗娜;叶文玲;陈海燕;何冰
单  位：
安徽农业大学资源与环境学院
关键词：
耐盐菌;16S rDNA;生长特性;苯酚;镉
摘  要：
为了研究高盐环境下菌株生长情况及对污染物的耐性特征,从危险废物填埋场渗滤液中筛选得到一株耐盐菌,编号为WS-1。通过对其形态进行观察,以及革兰氏染色和16S rDNA基因序列分析,可以鉴定该菌株为葡萄球菌属(Staphylococcus sp.)。通过调节培养基的盐度、pH,研究其对WS-1生长的影响,并考察WS-1对苯酚和镉的耐受性。确定WS-1在盐质量分数为5%～10%、pH为7.0、温度30℃时生长最好。WS-1在苯酚浓度0～1 000mg·L~(-1)的范围内均可很好生长,低浓度苯酚能促进菌株生长,高浓度苯酚会对菌株生长产生抑制作用,72 h内,菌株对苯酚的降解率可达38%左右。在Cd~(2+)浓度在1～5 mg·L~(-1)时,WS-1生长不受影响,当Cd~(2+)浓度达到10 mg·L~(-1),抑制作用明显,延长WS-1生长周期。低浓度时WS-1对Cd~(2+)去除率在90%左右,高浓度Cd~(2+)去除率在40%左右,效果不明显。WS-1可为高盐度含有机废物和重金属废水生物处理提供菌株资源。
译  名：
Screening, identification and tolerance to phenol and cadmium of a salt-tolerant strain
作  者：
LIU Yalou;FAN Ting;LIU Ru;PAN Dandan;GUO Yihui;LUO Na;YE Wenling;CHEN Haiyan;HE Bing;Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention,School of Resources and Environment,Anhui Agricultural University;
关键词：
salt-tolerant bacteria;;16S rDNA;;growth characteristics;;phenol;;cadmium
摘  要：
In order to study the growth of a strain and its tolerance to pollutants in high salt environment, a salt-tolerant strain was screened from a hazardous waste landfill leachate, numbered WS-1. By observing its morphology, as well as Gram staining and 16 S rDNA gene sequence analysis, the strain was identified as Staphylococcus sp. Effects of pH and salinity on the growth and the tolerance to phenol and cadmium of strain WS-1 were investigated, the results showed that the optimum conditions of WS-1 were salinity 5%～10%, pH 7.0, 30°C.The strain WS-1 could grow well in the range of phenol concentration 0-1000 mg·L-1, which growth was promoted at low-concentration and inhibited at high-concentration of phenol. The degradation rate of phenol by WS-1 was at about 38% in 72 h.When the concentration of Cd~(2+) was 1～5 mg·L~(-1), the growth of WS-1 was not affected. However, at high concentration of Cd~(2+)(10 mg·L~(-1)), the inhibitory effect was obvious and the growth cycle was prolonged of WS-1. At low concentration, the removal rate of Cd~(2+) was about 40%, at high concentration, the removal rate of Cd~(2+) was about 40% which not obvious. So, WS-1 shows the potential of application in biological treatment of high salinity wastewater including organic and heavy metal pollutions.

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