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当前位置: 首页 > 文章 > 3株耐盐细菌对萘、菲、惹烯和苯并[α]芘的降解性能 大连海洋大学学报 2018,33 (3) 307-315
Position: Home > Articles > Biodegradation of naphthalene,phenanthrene,retene and benzpyrene by 3 strains of halo tolerant bacteria Journal of Dalian Ocean University 2018,33 (3) 307-315

3株耐盐细菌对萘、菲、惹烯和苯并[α]芘的降解性能

作  者：
陈弘昊;李作扬;陈泉睿;何洁;赵欢;王斌
单  位：
大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室;大连海洋大学海洋科技与环境学院
关键词：
耐盐菌;多环芳烃;葡萄糖;共代谢
摘  要：
为研究3株耐盐细菌(S1 Microbacterium sp.、G12 Zhihengliuella sp.和Y3 Pseudomon putida)对多环芳烃的利用性能,分别测定其在以萘、菲、惹烯和苯并[α]芘为唯一碳源并添加不同浓度葡萄糖(0、0.5、1.0、1.5 g/L)的无机盐培养基中的生长情况,采用气质联用(GC-MS)技术测定了3株菌在上述培养基中作用7 d后对4种多环芳烃(PAHs)的降解性能,同时测定出3株菌的生长量并计算出单位细胞的降解效率。结果表明:3株菌均能够利用4种PAHs作为碳源,且在无糖的萘-无机盐培养基的中生长量高于其他3种PAHs-无机盐培养基,在萘、菲、惹烯-无机盐培养基的生长量均与含糖量成正比,但0.5、1.0、1.5 g/L葡萄糖组间无显著性差异(P>0.05);添加1.0 g/L葡萄糖时,3株菌对4种PAHs的降解率均可达到最高值,对萘的降解率分别提高了44.06%(S1)、70.56%(Y3)和50.98%(G12),对菲的降解率分别提高了49.66%(S1)、45.87%(Y3)和38.29%(G12),对惹烯的降解率分别提高了66.13%(S1)、61.31%(Y3)和56.20%(G12),对苯并[α]芘的降解率分别提高了69.42%(S1)、65.79%(Y3)和65.01%(G12)。研究表明,3株菌对4种PAHs单个细胞降解速率均随葡萄糖浓度的增加而大幅度降低,呈剂量反比关系。
译  名：
Biodegradation of naphthalene,phenanthrene,retene and benzpyrene by 3 strains of halo tolerant bacteria
作  者：
CHEN Hong-hao;LI Zuo-yang;CHEN Quan-rui;HE Jie;ZHAO Huan;WANG Bin;Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University;College of Marine Science and Environment,Dalian Ocean University;
关键词：
halo tolerant bacterium;;polycyclic aromatic hydrocarbons(PAHs);;glucose;;co-metabolism
摘  要：
The growth of three salt-tolerant bacterial strains( Microbacterium sp. S1,Zhihengliuella sp. G12 and Pseudomon asputida Y3) was investigated in inorganic salt media containing naphthalene,phenanthrene,retene or benzo [α] pyren as the sole carbon source and supplement with glucose at a concentration of 0,0.5,1.0,and 1.5 g/L for 7 d and the degradation of polycyclic aromatic hydrocarbons( PAHs) was analyzed by gas chromatography mass spectrometry( GC-MS) in order to probe into the degradation capabilities of three salt-tolerant bacterial strains for different PAHs. The results showed that all of the strains utilized the four kinds of PAHs as carbon sources,with the best growth in the naphthalene containing inorganic salt medium without glucose. Furthermore,the growth of these strains was found to be proportional to the concentration of glucose in the inorganic salt medium containing phenanthrene,retene or benzo [α] pyren,without significant difference among 0.5 g/L group,1 g/L group and 1.5 g/L group( P>0.05). The maximal PAHs degradation rate was observed in the three strains cultured in the inorganic salt medium containing 1 g/L glucose,with an increase by 44. 06%( S1),70. 56%( Y3) and50.98%( G12) for naphthalene degradation,49.66%( S1),45.87%( Y3) and 38.29%( G12) for phenanthrene degradation,66.13%( S1),61. 31%( Y3) and 56. 20%( G12) for retene degradation,and 69. 42%( S1),65.79%( Y3) and 65.01%( G12) for benzo [α]pyren degradation. However,the PAHs degradation efficiency of the single cell was significantly reduced with the increase in glucose concentration. The findings indicate that co-metabolism effect might be involved in the degradation process of the four PAHs by the three strains.

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