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Position: Home > Articles > Determination of Volatile Phenols in Maotai-Flavor Liquors by HPLC-FLD FOOD SCIENCE 2012,33 (24) 239-243

高效液相色谱法测定酱香型白酒中挥发性酚类物质

作  者：
寻思颖;董睿;彭黔荣;冯永渝;杨敏;孙棣;耿平兰
单  位：
贵州大学化学与化工学院;国家酒类及饮料产品质量监督检验中心;贵州中烟工业有限责任公司贵阳卷烟厂
关键词：
高效液相色谱;挥发性酚;β-环糊精;酱香型白酒
摘  要：
利用酚类物质的荧光特性和手性拆分剂β-环糊精拆分重叠峰,建立带荧光检测-高效液相色谱仪法测定酱香型白酒中10种挥发性酚类物质含量的方法,色谱条件如下:色谱柱,Synergi 4u Hydro-RP 80A(150mm×4.6mm,4μm);荧光检测器波长:Ex=270nm,Em=315nm;流动相A:冰醋酸-β-环糊精的水溶液(1:100,V/V);流动相B:冰醋酸-乙腈-β-环糊精的水溶液(1:40:60,V/V),梯度洗脱分析。该法对10种挥发性酚类化合物测定的回收率为83.8%～101.6%,相对标准偏差为0.9%～2.5%,检出限为0.0025～0.0506μg/mL。说明该方法准确度、灵敏度和重复性好。
译  名：
Determination of Volatile Phenols in Maotai-Flavor Liquors by HPLC-FLD
作  者：
XUN Si-ying1,DONG Rui2,PENG Qian-rong3,FENG Yong-yu1,YANG Min2,*,SUN Di1,GENG Ping-lan1(1.National Centre of Quality Supervision and Detection for Wine and Beverages,Guiyang 550004,China;2.School of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550003,China;3.Guiyang Cigarettes Factory,China Tobacco Guizhou Industrial Co.Ltd.,Guiyang 550003,China)
关键词：
HPLC;volatile phenols;β-cyclodextrin;Maotai-flavor liquors
摘  要：
A high-performance liquid chromatography with fluorescence detector(HPLC-FLD) method for the determination of volatile phenols in Maotai-flavor liquors was established.This method was based on the fluorescent nature of volatile phenols and the separation of overlapped peaks with β-cyclodextrin as a resolving agent.The chromatographic separation was achieved on Synergi 4u Hydro-RP 80A column(150 mm × 4.6 mm,4 μm) using acetic acid-β-cyclodextrin aqueous solution of(1:100,V/V) as mobile phase A and acetic acid-acetonitrile-β-cyclodextrin aqueous solution(1:40:60,V/V) by gradient elution.The excitation and emission wavelengths were set as 270 nm and 315 nm,respectively.Average recoveries of 10 volatile phenols in liquors were 83.8%–101.6% with RSDs ranging from 0.9% to 2.5%.The limits of detection of this method were 0.0025–0.0506 μg/mL.These results indicated that this method was accurate,sensitive and repeatable.

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