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Position: Home > Articles > Diagnosis of the Mixed Infection with PRRSV and Staphylococcus Guizhou Journal of Animal Husbandry & Veterinary Medicine 2018,42 (6) 32-34

猪蓝耳病与葡萄球菌病混合感染的诊断

作  者:
王云峰;贺欣薇;胡安东;杨颖;文明;张霞;皮泉
单  位:
贵州省动物疫病预防控制中心;贵州大学动物科学学院
关键词:
猪蓝耳病病毒;金黄色葡萄球菌;细菌分离鉴定;PCR检测
摘  要:
为确诊某养猪场死亡病例的病因,采集病死猪内脏组织进行细菌分离鉴定和猪瘟病毒、猪伪狂犬病病毒、猪蓝耳病病毒PCR检测。结果:(1)送检猪肝脏样本分离培养出呈金黄色的菌落,染色镜检可见菌体呈葡萄串状排列的革兰氏阳性球菌,经生化试验鉴定为金黄色葡萄球菌;(2)送检猪组织样本检出猪蓝耳病病毒特异性DNA片段,未检出猪瘟病毒和猪伪狂犬病病毒特异性DNA片段。综合诊断为猪蓝耳病病毒与金黄色葡萄球菌混合感染。
译  名:
Diagnosis of the Mixed Infection with PRRSV and Staphylococcus
作  者:
Wang Yunfeng;He Xinwei;Hu ANDong;Yang Ying;Wen Ming;Zhang Xia;Pi Quan;College of Animal Science,Guizhou University;Guizhou Centre for Prevention and Control of Animal Disease;
关键词:
PRRSV;;Staphylococcus aureus;;Bacterial Isolation and Identification;;PCR Detection
摘  要:
To confirm the death cause of the pigs in a pig farm,the pathogen was analyzed by isolation and identification of bacteria and PCR detection of CSFV and PRV and PRRSV respectively.Results:(1) the results showed that the golden yellow bacterial colonies could be grown form liver samples of the dead pigs,and the bacteria were identified as Staphylococcus aureus by microscopic examination after staining and biochemical activity.(2) By PCR or RT-PCR detection for viral nucleic acid from the tissues of the dead pigs,the specific DNA fragments of PRRSV was found but CSFV and PRV.These results suggested that the dead pigs were the mixed infection with PRRSV and Staphylococcus aureus.

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