当前位置: 首页 > 文章 > 具有不同胚胎发生及再生能力的橡胶愈伤组织其差异表达cDNA序列及组织学特性的鉴定 陕西林业科技 2008 (4) 184-192
Position: Home > Articles > Identification of Differentially Expressed cDNA Sequences and Histological Characteristics of Hevea Brasiliensis Calli in Relation to their Embryogenic and Regenerative Capacities Shaanxi Forest Science and Technology 2008 (4) 184-192

具有不同胚胎发生及再生能力的橡胶愈伤组织其差异表达cDNA序列及组织学特性的鉴定

作  者:
田郎
单  位:
中国热带农业科学院橡胶研究所
关键词:
体细胞胚胎发生;分子标记;橡胶树;差异显示技术
摘  要:
来自巴西橡胶树同一基因型的不同松散型愈伤组织系可能会具有不同的胚胎发生及再生潜力,但从外观上却很难对其加以区分。为了深入揭示不同愈伤组织系之间所存在的差异,我们对5个具有不同胚胎发生及再生潜力的愈伤组织系进行了组织及分子学比较研究。试验中我们采用差异显示(DD-RT)技术对诱导期间差异表达的基因进行了分析。在胚性再生型愈伤组织系(ER)诱导期间共鉴定出28个差异表达的cDNA。此外还发现,ER系中胚胎发生结节(胚性结节)的形成要早于胚性非再生型愈伤组织系(ENR),而非胚性愈伤组织系(NE)中则根本不形成胚性结节。以上28个cDNAs中,有5个可用于诱导之前区分具有不同胚胎发生潜力的愈伤组织系,从而可以对橡胶树松散型愈伤组织的胚胎发生潜力作出提前判断。
译  名:
Identification of Differentially Expressed cDNA Sequences and Histological Characteristics of Hevea Brasiliensis Calli in Relation to their Embryogenic and Regenerative Capacities
作  者:
TIAN Lang (Rubber Research Institute Chinese Academy of Tropical Agriculture Science,danzhou,Hainan,571737)
关键词:
Somatic embryogenesis;molecular marker;rubber tree;differential display technique
摘  要:
Different friable Hevea callus lines from the same genotype can display different embryogenic and regenerative potentials.These lines can not be distinguished on the basis of macroscopic criteria.A histological and molecular study was undertaken to characterize the differences existing between five callus lines with different potentials.The genes differentially expressed during induction were analyzed using the differential display(DD-RT) technique.Twenty-eight cDNAs were found to be differentially expressed during induction in the embryogenic regenerating line(ER).Embryogenic nodules were formed earlier in the ER lines than in the embryogenic non-regenerating line and were completely absent in the non-embryogenic line.Of these 28 cDNAs,five could be used to distinguish between calli prior to induction,thereby enabling an early diagnosis of friable Hevea callus embryogenic potential.

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