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Position: Home > Articles > Study on Determination of Polydextrose Content by HPLC FOOD SCIENCE 2007,28 (8) 422-425

高效液相色谱法测定聚葡萄糖含量的研究

作  者:
康琪;朱若华
单  位:
首都师范大学化学系
关键词:
高效液相色谱;水溶性膳食纤维;聚葡萄糖
摘  要:
用Waters Ultrahydrogel 250(4.6×300mm)色谱柱,以水作流动相,示差折光检测,进样量20μl,在流速0.5ml/min下,直接测定样品中的可溶性膳食纤维聚葡萄糖含量和平均分子量。建立了分子量与保留时间的关系方程,用于样品中聚葡萄糖的分子量测定。聚葡萄糖浓度在0.199mg/ml~3.98mg/ml范围内与峰面积呈良好的线性关系,方法RSD小于1.00%,回收率103%~110%(RSD=1.91%)之间。结果显示,该方法是简单、准确、可靠的,适合于食品中水溶性膳食纤维聚葡萄糖含量的测定。
译  名:
Study on Determination of Polydextrose Content by HPLC
作  者:
KANG Qi,ZHU Ruo-hua (Department of Chemistry, Capital Normal University, Beijing 100037, China)
关键词:
HPLC;soluble dietary fiber;polydextrose
摘  要:
The HPLC was run on an Ultrahydrogel 250 column (7.8×300 mm) with refractive index (RI) detector for determinations of content and average molecular weight of polydextrose in some samples. Distilled water was used as the mobile phase at a flow rate of 0.5 ml/min. The correlation equation was established between molecular weight and retention time and applied to estimate the molecular weight of polydextrose samples. The calibration curve was linear over the polydextrose concentration range of 0.199~3.98 mg/ml, with a correlation coefficient of r=0.9998. The polydextrose mass recovery ranged from 103% to 110% with a RSD of 1.91%. The method is found to be simple, reproducible and reliable, and thus ideally suitable for the analysis of polydextrose in food product.

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