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当前位置: 首页 > 文章 > 参薯组织培养的多因子正交试验研究 热带农业科学 2018,38 (5) 43-48
Position: Home > Articles > Multi-factorial Orthogonal Experiment on the Tissue Culture of Dioscorea alata Chinese Journal of Tropical Agriculture 2018,38 (5) 43-48

参薯组织培养的多因子正交试验研究

作  者：
蒙真铖;苏翠;杨曦;孔得信
关键词：
参薯;多因子;正交试验;组织培养
摘  要：
以参薯茎段为研究材料,使用0.1%HgCl2进行不同消毒时间的消毒处理,并应用正交试验设计对参薯无菌苗的诱导、 生根培养进行研究.结果表明:参薯茎段以0.1%HgCl2消毒7 min效果较好,污染率低,存活率及发芽率较高;最佳诱导培养基为:MS+6-BA 0.5 mg/L+GA30.1 mg/L+AC 1.0 g/L+蔗糖30 g/L;最佳生根培养基为:1/2 MS+NAA 0.5 mg/L+IBA 0.5 mg/L+蔗糖25 g/L+AC 0.5 g/L;添加0.5~1 g/L的AC能有效减小参薯组培苗的褐化.
译  名：
Multi-factorial Orthogonal Experiment on the Tissue Culture of Dioscorea alata
作  者：
MENG Zhencheng;SU Cui;YANG Xi;KONG Dexin;Yunnan Honghe Research Institute of Tropical Agriculture;
关键词：
Dioscorea alata;;many factors;;orthogonal test;;tissue culture
摘  要：
Vine stems of Dioscorea alata were cut into segments and disinfected with 0.1% Hg Cl2 for different durations for microprogation by tissue culture, and an orthogonal experiment was designed and conducted for multiplication and root growth of the disinfected stem segments. The results showed that the stem segments of D. alata was better in disinfection when treated with 0.1% Hg Cl2 for 7 min, and had a lower contamination rate as well as a higher survival rate and a sprouting rate. The best medium for inducing and multiplication was MS+6-BA 0.5 mg/L+GA30.1 mg/L+AC 1.0 g/L+sucrose 30 g/L; the best rooting medium was 1/2 MS+NAA 0.5 mg/L+IBA 0.5 mg/L+sucrose 25 g/L+AC 0.5 g/L; supplement of0.5~1 g/L of AC in the medium could effectively reduce the browning of the tissue culture plants.

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