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当前位置: 首页 > 文章 > 参薯多糖检测及其体外抗氧化能力研究 河南农业科学 2011,40 (11) 113-116
Position: Home > Articles > Determination and Antioxidant Activity in vitro of Polysaccharides from Dioscorea alata Journal of Henan Agricultural Sciences 2011,40 (11) 113-116

参薯多糖检测及其体外抗氧化能力研究

作  者：
裴会鹏;黄俊;吴凯旋;杨培君
单  位：
陕西理工学院生物科学与工程学院
关键词：
参薯;多糖;体外抗氧化作用
摘  要：
以参薯(Dioscorea alata L.)块茎为研究材料,采用热水浸提、Savage法除蛋白、乙醇沉淀及有机溶剂纯化的方法,获得参薯粗多糖。通过蒽酮-硫酸显色法测定参薯的多糖含量,研究了参薯粗多糖体外对羟自由基(.OH)和过氧化氢(H2O2)的清除能力。结果表明:参薯多糖含量高达19.17%,参薯粗多糖对羟自由基(.OH)和过氧化氢(H2O2)具有一定的清除活性,抗氧化能力与多糖浓度之间存在良好的相关性。当粗多糖质量浓度分别达到9.0mg/mL和0.5mg/mL时,对羟自由基和过氧化氢的清除率可达到100%。参薯多糖具有较好的体外抗氧化作用,是一种具有开发价值的天然抗氧化剂。
译  名：
Determination and Antioxidant Activity in vitro of Polysaccharides from Dioscorea alata
作  者：
PEI Hui-peng,HUANG Jun,WU Kai-xuan,YANG Pei-jun(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China)
关键词：
Dioscorea alata L.;Polysaccharide;Antioxidant activity in vitro
摘  要：
The tuber of Dioscorea alata was used as test material to determine the polysaccharide and the antioxidant activity in vitro.The methods contained hot water extraction,Sevage removal protein,precipitation by ethanol,purifying polysaccharide by organic solvents,producing polysaccharide of D.alata.Moreover,the antioxidant activities in vitro of D.alata polysaccharide on hydroxyl radicals(·OH) and hydrogen peroxide(H2O2) were researched with anthrone-sulfuric acid colorimetric.The results indicated that the content of D.alata polysaccharide was 19.17% and had a clearing ability on ·OH and H2O2,the antioxidant activity was significantly correlated with its concentration.The polysaccharide completely scavenged the hydroxyl and hydrogen peroxide free radicals when its mass concentration was 9.0 mg/mL and 0.5 mg/mL respectively.D.alata can be used as a resource of plant active polysaccharides with a strong antioxidant effect,and can be exploited as a natural antioxidant.

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