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当前位置: 首页 > 文章 > 化学发光酶联免疫法检测苏丹红Ⅰ 食品科学 2015,36 (12) 209-212
Position: Home > Articles > Determination of Sudan Ⅰ by Chemiluminescent Enzyme Immunoassay FOOD SCIENCE 2015,36 (12) 209-212

化学发光酶联免疫法检测苏丹红Ⅰ

作  者：
范艳;孟玮;朱立鑫;刘仁荣;许龙;裘雪梅;杨帆帆
单  位：
江西科技师范大学生命科学学院
关键词：
苏丹红Ⅰ;化学发光酶联免疫分析;酶联免疫吸附分析
摘  要：
为检测食品中苏丹红Ⅰ残留,建立间接竞争化学发光酶联免疫分析(chemiluminescent enzyme immunoassay,CLEIA)法。通过优化包被抗原中本抗原与载体物质的量比、包被抗原质量浓度、抗体稀释比例,建立竞争抑制曲线。线性范围为0.156~5 ng/m L,最低检测限为0.078 9 ng/m L,IC50为0.679 ng/m L。CLEIA回收率为75.08%~112.18%,变异系数为8.89%~15.61%;通过与酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法进行比较,在相同抗原抗体质量浓度条件下,CLEIA法测定的IC50较ELISA方法降低30%,具有较高的灵敏度。
译  名：
Determination of Sudan Ⅰ by Chemiluminescent Enzyme Immunoassay
作  者：
FAN Yan;MENG Wei;ZHU Lixin;LIU Renrong;XU Long;QIU Xuemei;YANG Fanfan;College of Life Science, Jiangxi Science and Technology Normal University;
关键词：
Sudan Ⅰ;;chemiluminescent enzyme immunoassay(CLEIA);;enzyme-linked immunosorbent assay(ELISA)
摘  要：
An indirect competitive chemiluminescent enzyme immunoassay(CLEIA) was developed for the detection of Sudan Ⅰ in food products. The CLEIA conditions including molar ratio of coating antigen to carrier, antigen concentration, and dilution ratio of antibody were optimized. In the standard curve of the optimized CLEIA, the linear range was 0.156– 5 ng/m L, the half maximal inhibitory concentration(IC50) was 0.679 ng/m L and the limit of detection(LOD) was 0.078 9 ng/m L. The CLEIA showed good recoveries with spiked chili powder ranging from 75.08% to 112.18%. Compared with ELISA method, the IC50 values of CLEIA was reduced by 30%. The proposed method has a high sensitivity.

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